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This volume contains papers presented at the Ninth International
Conference on Second Messengers and Phosphoproteins. Written by
leading scientists - including two Nobel Laureates - the papers
highlight contemporary advances in the rapidly evolving field of
signal transduction. The findings presented are of vital
significance to researchers in virtually all biomedical fields,
including pharmacology, molecular biology, cell biology,
biochemistry, the neurosciences, and physiology.
The contributors offer new insights into fundamental cell
signalling mechanisms and explore the role of these mechanisms in
physiological and pathophysiological responses in a variety of
systems. Coverage includes many topics that are currently under
intensive study, such as growth factors and special signalling
systems; protein phosphatases and metabolic pathways; calcium and
ion channels; cyclic GMP and cyclic AMP; and receptors and G
proteins.
This volume emphasizes methods for the assay, purification, and
characterization of adenylyl cyclases, guanine nucleotide-dependent
regulatory proteins (G proteins), and guanylyl cyclases.
Key Features
* Adenylyl cyclase: determination of activity
* Preparation of materials useful in purification of components of
hormonally responsive cyclase systems
* Purification guanine nucleotide-dependent regulatory
proteins
* Purification and characterization of g proteins: labeling and
quantitating of G proteins
* Reconstitution guanylyl cyclase: assay
* Preparation of materials used for study of the cyclase
* Purification
* Regulation
The critically acclaimed laboratory standard, Methods in
Enzymology, is one of the most highly respected publications in the
field of biochemistry. Since 1955, each volume has been eagerly
awaited, frequently consulted, and praised by researchers and
reviewers alike. The series contains much material still relevant
today - truly an essential publication for researchers in all
fields of life sciences.
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