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Electroporation gene therapy, or gene electrotransfer, has evolved
greatly over the last few decades as a result of the remarkable
progress in genetic sequencing, gene array analysis, gene cloning,
gene expression detection, DNA manufacture and discovery and
synthesis of siRNA. Electroporation Protocols: Preclinical and
Clinical Gene Medicine, Second Edition provides in-depth knowledge
on the delivery of naked DNA and small-interfering RNA (siRNA) to
the targeted cells, tissues, and animals for prevention and
treatment of disease. It builds on the success of the first edition
and on the progress made in siRNA delivery and DNA vaccines for
large animals as well as discovery of electroporation applications
for the fragile tissues and for internal organs. Written in the
successful Methods in Molecular Biology series format, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible protocols and notes on troubleshooting and avoiding
known pitfalls. Authoritative and easily accessible,
Electroporation Protocols: Preclinical and Clinical Gene Medicine,
Second Edition aims to provide not only comprehensive coverage of
the basic theory and practical application of electroporation siRNA
therapy, gene therapy, and vaccine, but also elaborates on the most
current views from the experts in this field, serving as an
invaluable resource for investigators both in and outside of this
field.
Electroporation gene therapy, or gene electrotransfer, has evolved
greatly over the last few decades as a result of the remarkable
progress in genetic sequencing, gene array analysis, gene cloning,
gene expression detection, DNA manufacture and discovery and
synthesis of siRNA. Electroporation Protocols: Preclinical and
Clinical Gene Medicine, Second Edition provides in-depth knowledge
on the delivery of naked DNA and small-interfering RNA (siRNA) to
the targeted cells, tissues, and animals for prevention and
treatment of disease. It builds on the success of the first edition
and on the progress made in siRNA delivery and DNA vaccines for
large animals as well as discovery of electroporation applications
for the fragile tissues and for internal organs. Written in the
successful Methods in Molecular Biology series format, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible protocols and notes on troubleshooting and avoiding
known pitfalls. Authoritative and easily accessible,
Electroporation Protocols: Preclinical and Clinical Gene Medicine,
Second Edition aims to provide not only comprehensive coverage of
the basic theory and practical application of electroporation siRNA
therapy, gene therapy, and vaccine, but also elaborates on the most
current views from the experts in this field, serving as an
invaluable resource for investigators both in and outside of this
field.
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