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This volume covers protocols related to both pluripotent and
somatic stem cells, including the ethical procurement of tissues
and cells for the provision of "seed stock," standardized methods
for deriving hESCs and iPSCs, isolating mesenchymal stem cells,
cell culture and cryopreservation, in addition to quality assurance
and information management. Stem Cell Banking: Concepts and
Protocols aims to contribute to the development of this field by
providing information that is essential to establishing a bona fide
stem cell bank. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Stem Cell Banking: Concepts and
Protocols is a valuable resource for stem cell scientists and
novices to the field, and will help strengthen and maximize their
use of existing and future stem cell resources.
This volume covers protocols related to both pluripotent and
somatic stem cells, including the ethical procurement of tissues
and cells for the provision of "seed stock," standardized methods
for deriving hESCs and iPSCs, isolating mesenchymal stem cells,
cell culture and cryopreservation, in addition to quality assurance
and information management. Stem Cell Banking: Concepts and
Protocols aims to contribute to the development of this field by
providing information that is essential to establishing a bona fide
stem cell bank. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Stem Cell Banking: Concepts and
Protocols is a valuable resource for stem cell scientists and
novices to the field, and will help strengthen and maximize their
use of existing and future stem cell resources.
This volume explores the latest developments and contributions to
the field of 3D bioprinting, and discusses its use for quality
R&D and translation. The chapters in this book are divided into
two parts: Part one covers generic themes in bioprinting to
introduce novice readers to the field, while also providing experts
with new and helpful information. Part two discusses protocols used
to prepare, characterize, and print a variety of biomaterials,
cells, and tissues. These chapters also emphasize methods used for
printing defined and humanized constructs suitable for human tissue
modelling in research and applicable to clinical product
development. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Cutting-edge and
comprehensive, 3D Bioprinting: Methods and Protocols is a valuable
resource for researchers and bioprinting laboratories/facilities
interested in learning more about this rapidly evolving technology.
This volume explores the latest developments and contributions to
the field of 3D bioprinting, and discusses its use for quality
R&D and translation. The chapters in this book are divided into
two parts: Part one covers generic themes in bioprinting to
introduce novice readers to the field, while also providing experts
with new and helpful information. Part two discusses protocols used
to prepare, characterize, and print a variety of biomaterials,
cells, and tissues. These chapters also emphasize methods used for
printing defined and humanized constructs suitable for human tissue
modelling in research and applicable to clinical product
development. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Cutting-edge and
comprehensive, 3D Bioprinting: Methods and Protocols is a valuable
resource for researchers and bioprinting laboratories/facilities
interested in learning more about this rapidly evolving technology.
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