This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.

This volume offers an up-to-date overview of biotechnologically oriented barley research. It is structured into two major sections: the first focusing on current agricultural challenges and approaches to barley improvement, and the second providing insights into recent advances in methodology. Leading scientists highlight topics such as: the global importance of barley; genetic diversity and genebanks; domestication; shoot and inflorescence architecture; reproductive development; mineral nutrition; photosynthesis and leaf senescence; grain development; drought tolerance; viral and fungal pathogens; phytophagous arthropods; molecular farming; sequence resources; induced genetic variation and TILLING; meiotic recombination; Hordeum bulbosum; genome-wide association scans; genomic selection; haploid technology; genetic engineering; and whole plant phenomics. Providing comprehensive information on topics ranging from fundamental aspects to specific applications, this book offers a useful resource for scientists, plant breeders, teachers and advanced students in the fields of molecular and plant cell biology, plant biotechnology, and agronomy.

This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.