This third edition expands on the previous editions with updated and new chapters on protein chromatography. Chapters detail protein stability and storage, avoiding proteolysis, protein quantitation methods, generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.   Authoritative and cutting-edge, Protein Chromatography: Methods and Protocols, Third Edition aims to provide commonly used methods and new approaches to help both new researchers and experts expand their knowledge. 

While living in Japan, John Milne (1850 1913) sought to study the 1880 Yokohama earthquake, soon realising that scientists lacked the proper tools. Aided by colleagues, he went on to develop the necessary instrumentation, and by 1896 he had built the first seismograph capable of recording major earthquakes in any part of the world. His textbook Earthquakes and Other Earth Movements (also reissued in this series) had appeared in 1886. In this follow-up work, published in 1898, Milne continues to discuss the nature of earthquakes, the methods and equipment needed to investigate them, and how to apply this knowledge to construction. He references the research, hypotheses and formulae of modern scientists, also noting in passing the suggestions made by earlier authors on the causes of seismic activity. The text is accompanied by many diagrams, especially of experimental apparatus, and several photographs illustrate damaged buildings and bridges."

John Milne (1850 1913) was a professor of mining and geology at the Imperial College of Engineering, Tokyo. While living in Japan, Milne became very interested in seismology, prompted by a strong seismic shock he experienced in Tokyo in 1880. Sixteen years later Milne and two colleagues completed work on the first seismograph capable of recording major earthquakes. This book, originally published in London in 1886, explains why earthquakes happen and what effects they have on land and in the oceans. As Milne points out, Japan provided him with 'the opportunity of recording an earthquake every week'. Starting with an introduction examining the relationship of seismology to the arts and sciences, the book includes chapters on seismometry, earthquake motion, the causes of earthquakes, and their relation to volcanic activity, providing a thorough account of the state of knowledge about these phenomena towards the end of the nineteenth century.

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Colonial, Neoclassical, Queen Anne, Craftsman-what distinguishes one architectural style from another? This unique book will allow readers to recognize the architectural features and style of virtually any house they encounter. Here, architect John Milnes Baker explains the historical, cultural, and technical influences that shaped each of these styles and many more. Organized in periods, from Early Colonial (1600-1715) to the Modern Movement (1920-60) and beyond, this tour of America's varied residential architecture is rendered in elevation drawings that precisely illustrate the key characteristics of each style. Nearly 25 years since the original publication of American House Styles, this updated edition includes a new preface and house styles from the mid-1990s to the present-from the rise and fall of the McMansion to energy-efficient, regionally influenced homes. The illustrations, now in color, are more delightful than ever in a new, larger format. This a must-have volume for anyone interested in architecture or adding a bit of style to their home.