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The critically acclaimed laboratory standard for more than forty
years, Methods in Enzymology is one of the most highly respected
publications in the field of biochemistry. Since 1955, each volume
has been eagerly awaited, frequently consulted, and praised by
researchers and reviewers alike. Now with more than 300 volumes
(all of them still in print), the series contains much material
still relevant today--truly an essential publication for
researchers in all fields of life sciences.
This new volume of "Methods in Enzymology" continues the legacy
of this premier serial by containing quality chapters authored by
leaders in the field. The volumecovers ghrelin, andhas chapters on
such topics as orphan gpcrs and methods for identifying their
ligands, ghrelin o-acyltransferase assays and inhibition, and
thermogenic characterization of ghrelin receptor null mice.
Contains quality chapters authored by leaders in the field Has
chapters on such topics as orphan gpcrs and methods for identifying
their ligands, ghrelin o-acyltransferase assays and inhibition, and
thermogenic characterization of ghrelin receptor null mice"
By combining the tools of organic chemistry with those of physical
biochemistry and cell biology, "Non-Natural Amino" "Acids" aims to
provide fundamental insights into how proteins work within the
context of complex biological systems of biomedical interest.
The critically acclaimed laboratory standard for 40 years, "Methods
in Enzymology" is one of the most highly respected publications in
the field of biochemistry. Since 1955, each volume has been eagerly
awaited, frequently consulted, and praised by researchers and
reviewers alike. With more than 400 volumes published, each
"Methods in Enzymology" volume presents material that is relevant
in today's labs -- truly an essential publication for researchers
in all fields of life sciences.
Demonstrates how the tools and principles of chemistry combined
with the molecules and processes of living cells can be combined to
create molecules with new properties and functions found neither in
nature nor in the test tube
Presents new insights into the molecular mechanisms of complex
biological and chemical systems that can be gained by studying the
structure and function of non-natural molecules
Provides a "one-stop shop" for tried and tested essential
techniques, eliminating the need to wade through untested or
unreliable methods
Antisense technology is the ability to manipulate gene expression
within mammalian cells providing powerful experimental approaches
for the study of gene function and gene regulation. For example,
methods that inhibit gene expression permit studies which probe the
normal function of a specific product within a cell. Such
methodology can be used in many disciplines such as pharmacology,
oncology, genetics, cell biology, developmental biology, molecular
biology, biochemistry, and neurosciences. This volume will be a
truly important tool in biomedical-oriented research.
The critically acclaimed laboratory standard for more than forty
years, Methods in Enzymology is one of the most highly respected
publications in the field of biochemistry. Since 1955, each volume
has been eagerly awaited, frequently consulted, and praised by
researchers and reviewers alike. Now with more than 300 volumes
(all of them still in print), the series contains much material
still relevant today--truly an essential publication for
researchers in all fields of life sciences.
This volume supplements Volumes 63, 64, 87, and 249 of Methods in
Enzymology. These volumes provide a basic source for the
quantitative interpretation of enzyme rate data and the analysis of
enzyme catalysis. Among the major topics covered are Engergetic
Coupling in Enzymatic Reactions, Intermediates and Complexes in
Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds,
Transition State Determination, and Inhibitors.
The critically acclaimed laboratory standard for more than forty
years, Methods in Enzymology is one of the most highly respected
publications in the field of biochemistry. Since 1955, each volume
has been eagerly awaited, frequently consulted, and praised by
researchers and reviewers alike. Now with more than 300 volumes
(all of them still in print), the series contains much material
still relevant today--truly an essential publication for
researchers in all fields of life sciences.
The critically acclaimed laboratory standard for more than forty
years, Methods in Enzymology is one of the most highly respected
publications in the field of biochemistry. Since 1955, each volume
has been eagerly awaited, frequently consulted, and praised by
researchers and reviewers alike. More than 285 volumes have been
published (all of them still in print) and much of the material is
relevant even today-truly an essential publication for
researchersin all fields of life sciences.
Key Features
* Prokaryotic ABC Transporters
* Eukaryotic ABC Transporters
* Nonmammalian ABC Transport Systems
* Mammalian P-Glycoproteins
* Multidrug Resistance Associated Protein
* Cystic Fibrosis Transmembrane Conductance Regulator
* Sulfonylurea Receptor
* Intracellular ABC Transporters
The critically acclaimed laboratory standard for more than forty
years, Methods in Enzymology is one of the most highly respected
publications in the field of biochemistry. Since 1955, each volume
has been eagerly awaited, frequently consulted, and praised by
researchers and reviewers alike. More than 260 volumes have been
published (all of them still in print) and much of the material is
relevant even today--truly an essential publication for researchers
in all fields of life sciences.
Key Features
* Liquid chromatography
* Electrophoresis
* Mass spectrometry
This text concerns the computer-based design and modelling,
computational approaches and instrumental methods for elucidating
molecular mechanisms of protein folding. Ligand-acceptor
interactions are included in volumes 202 and 203 as are genetic and
chemical methods for the production of functional molecules
including antibodies and antigens, enzymes, receptors, nucleic
acids and polysaccharides and drugs.
This volume, as does Volume 176, provides a general background of
modern NMR techniques, with a specific focus on NMR techniques that
pertain to proteins and enzymology, and a "snapshot" of the current
state-of-the-art in NMR experimental techniques. These books enable
the reader to understand a given technique, to evaluate its
strengths and limitations, to decide which is the best approach,
and, finally, to design an experiment using the chosen technique to
solve a problem.
The critically acclaimed laboratory standard for more than forty
years, Methods in Enzymology is one of the most highly respected
publications in the field of biochemistry. Since 1955, each volume
has been eagerly awaited, frequently consulted, and praised by
researchers and reviewers alike. Now with more than 300 volumes
(all of them still in print), the series contains much material
still relevant today--truly an essential publication for
researchers in all fields of life sciences.
Recent advances in understanding the biological role of singlet
oxygen in the pathways of cellular responses to ultraviolet-A
radiation: its key position in photodynamical effects, and its
generation by photochemical (dark) reactions, e.g. by cells of the
immune system such as eosinophils and macrophages, are the focus of
this volume. The new methods and techniques responsible for the
rapid progress in this area are presented.
The critically acclaimed laboratory standard for more than forty
years, Methods in Enzymology is one of the most highly respected
publications in the field of biochemistry. Since 1955, each volume
has been eagerly awaited, frequently consulted, and praised by
researchers and reviewers alike. Now with more than 300 volumes
(all of them still in print), the series contains much material
still relevant today--truly an essential publication for
researchers in all fields of life sciences.
The critically acclaimed laboratory standard for more than forty
years, Methods in Enzymology is one of the most highly respected
publications in the field of biochemistry. Since 1955, each volume
has been eagerly awaited, frequently consulted, and praised by
researchers and reviewers alike. More than 270 volumes have been
published (all of them still in print) and much of the material is
relevant even today--truly an essential publication for researchers
in all fields of life sciences.
Key Features
* Crystal Growth
* Phase Determination
* Instrumentation and Data Collection
* Model Building
* Refinement
The critically acclaimed laboratory standard for forty years,
Methods in Enzymology is one of the most highly respected
publications in the field of biochemistry. Since 1955, each volume
has been eagerlyawaited, frequently consulted, and praised by
researchers and reviewers alike. More than 250 volumes have been
published (all of them still in print) and much of the material is
relevant even today--truly an essential publication for researchers
in all fields of life sciences.
Key Features
* Methods for the quantitation of apolipoproteins related to
cardiovascular disease
* Problems of lipoprotein heterogeneity and quantitation
* Apolipoproteins B-100 and B-48, timely because of current
interest in post-prandial lipoproteins and their contribution to
risk for atherosclerosis and coronary disease
This volume of Methods in Enzymology and its companion Volume 238
include molecular, biological, and biochemical methods for the
study of cell surface heterotrimeric G proteins (Volume 237) and
their effectors (Volume 238). Methods unique to signal transducing
G proteins and general techniques applied to the study of G protein
systems are covered.
This book is aimed at biochemists, geneticists, molecular
biologists, cell biologists, microbiologists, genetic engineers,
analytical chemists, biophysicists, and industrial researchers
working on protein products.;Of use to researchers who study
transcriptional control, protein-DNA recognition, DNA replication,
protein structure and function, and related topics, it aims to
provide an up-to-date and critical description of the chemical,
biochemical, biophysical, and genetic methods that are commonly
used in the study of DNA binding proteins.
This volume provides up-to-date, state-of-the-art methods for
determining the metal content of metalloenzymes, metalloproteins,
and other metallobiochemicals. It emphasizes the need for control
of contamination, techniques for handling and dealing with
contamination-sensitive molecules, and the absolute necessity of
quantitating metal content in systems inferred to contain metals by
indirect means.
The methods presented in this volume will enable the reader to
design effective strategies for the expression of cloned genes and
cDNAs and will prove useful in solving the majority of expression
problems one is likely to encounter.
The critically acclaimed laboratory standard, Methods in
Enzymology, is one of the most highly respected publications in the
field of biochemistry. Since 1955, each volume has been eagerly
awaited, frequently consulted, and praised by researchers and
reviewers alike. The series contains much material still relevant
today - truly an essential publication for researchers in all
fields of life sciences.
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