This volume explores various approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Each chapter provides comprehensive descriptions and guidelines on how to perform innovative experiments in CTC research. Included are protocols for capture of CTCs via filtration and density gradient centrifugation; microfluidic and immunomagnetic separation of CTCs; detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, and flow cytometry; assays designed for genomic characterization and functional analyses of CTCs, and many more. Written in the highly successful Methods in Molecular Biology series format, the chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Circula ting Tumor Cells: Methods and Protocols is a valuable resource for laboratory researchers and clinicians who are interested in furthering their studies on CTCs.

In this timely book, John Parkes demystifies the brewing process and explains in easy-to-follow terms how anyone can produce delicious beer with the help of just some basic equipment and a few key skills. Those new to home brewing will love the easy-to-follow instructions and the detailed explanations of the brewing process and anyone already adept at home brewing will be delighted by the original recipes. Made without unnecessary chemicals and additives, the beers featured here will appeal to anyone seeking a more self-sufficient lifestyle.

This volume examines the psychological basis of moral judgments and asks what theories of concepts apply to moral concepts. By combining philosophical reasoning and empirical insights from the fields of moral psychology, cognitive science, evolutionary psychology, and neuroscience, it considers what mental states not only influence, but also constitute our moral concepts and judgments. On this basis, Park proposes a novel pluralistic theory of moral concepts which includes three different cognitive structures and emotions. Thus, our moral judgments are shown to be a hybrid that express both cognitive and conative states. In part through analysis of new empirical data on moral semantic intuitions, gathered via cross-cultural experimental research, Park reveals that the referents of individuals' moral judgments and concepts vary across time, contexts, and groups. On this basis, he contends for moral relativism, where moral judgments cannot be universally true across time and location but only relative to groups. This powerfully argued text will be of interest to researchers, academics, and educators with an interest in cognitive science, moral theory, philosophy of psychology, and moral psychology more broadly. Those interested in ethics, applied social psychology, and moral development will also benefit from the volume.

There are many data communications titles covering design, installation, etc, but almost none that specifically focus on industrial networks, which are an essential part of the day-to-day work of industrial control systems engineers, and the main focus of an increasingly large group of network specialists.
The focus of this book makes it uniquely relevant to control engineers and network designers working in this area. The industrial application of networking is explored in terms of design, installation and troubleshooting, building the skills required to identify, prevent and fix common industrial data communications problems - both at the design stage and in the maintenance phase.
The focus of this book is 'outside the box'. The emphasis goes beyond typical communications issues and theory to provide the necessary toolkit of knowledge to solve industrial communications problems covering RS-232, RS-485, Modbus, Fieldbus, DeviceNet, Ethernet and TCP/IP. The idea of the book is that in reading it you should be able to walk onto your plant, or facility, and troubleshoot and fix communications problems as quickly as possible. This book is the only title that addresses the nuts-and-bolts issues involved in design, installation and troubleshooting that are the day-to-day concern of engineers and network specialists working in industry.
* Provides a unique focus on the industrial application of data networks
* Emphasis goes beyond typical communications issues and theory to provide the necessary toolkit of knowledge to solve industrial communications problems
* Provides the tools to allow engineers in various plants or facilities to troubleshoot and fix communications problems as quickly as possible

This volume examines the psychological basis of moral judgments and asks what theories of concepts apply to moral concepts. By combining philosophical reasoning and empirical insights from the fields of moral psychology, cognitive science, evolutionary psychology, and neuroscience, it considers what mental states not only influence, but also constitute our moral concepts and judgments. On this basis, Park proposes a novel pluralistic theory of moral concepts which includes three different cognitive structures and emotions. Thus, our moral judgments are shown to be a hybrid that express both cognitive and conative states. In part through analysis of new empirical data on moral semantic intuitions, gathered via cross-cultural experimental research, Park reveals that the referents of individuals' moral judgments and concepts vary across time, contexts, and groups. On this basis, he contends for moral relativism, where moral judgments cannot be universally true across time and location but only relative to groups. This powerfully argued text will be of interest to researchers, academics, and educators with an interest in cognitive science, moral theory, philosophy of psychology, and moral psychology more broadly. Those interested in ethics, applied social psychology, and moral development will also benefit from the volume.

With this volume, The University of California Center for New Racial Studies inaugurates a new book series with Routledge. Focusing on the shifting and contradictory meaning of race, The Nation and Its Peoples underscores the persistence of structural discrimination, and the ways in which "race" has formally disappeared in the law and yet remains one of the most powerful, underlying, unacknowledged, and often unspoken aspects of debates about citizenship, about membership and national belonging, within immigration politics and policy. This collection of original essays also emphasizes the need for race scholars to be more attentive to the processes and consequences of migration across multiple boundaries, as surely there is no place that can stay fixed-racially or otherwise-when so many people have been moving. This book is ideal as required reading in courses, as well as a vital new resource for researchers throughout the social sciences.

With this volume, The University of California Center for New Racial Studies inaugurates a new book series with Routledge. Focusing on the shifting and contradictory meaning of race, The Nation and Its Peoples underscores the persistence of structural discrimination, and the ways in which "race" has formally disappeared in the law and yet remains one of the most powerful, underlying, unacknowledged, and often unspoken aspects of debates about citizenship, about membership and national belonging, within immigration politics and policy. This collection of original essays also emphasizes the need for race scholars to be more attentive to the processes and consequences of migration across multiple boundaries, as surely there is no place that can stay fixed-racially or otherwise-when so many people have been moving. This book is ideal as required reading in courses, as well as a vital new resource for researchers throughout the social sciences.

Since the emergence of postmodern social theory, history has been haunted by predictions of its imminent end. Postmodernism has been accused of making historical research and writing untenable, encouraging the proliferation of revisionist histories, providing fertile ground for historical denial, and promoting the adoption of a mournful view of the past. This provocative book re-examines the nature of the alleged "threat" to history posed by postmodernism, and explores the implications of postmodern social theory for history as curriculum. Interrupting History will be of interest to curricularists and critical pedagogues around the globe, and to history educators at all levels of education. Making an important contribution to the struggle for critical and effective histories, it is a must-read text for those studying or teaching history today.

For over a century, teachers, parents, and school leaders have lamented a loss of 'discipline' in classrooms. Caught between guidance approaches on the one hand and a call for zero tolerance on the other, current debates rarely venture beyond the terrain of implementation strategies. This book aims to reinvigorate thinking on 'discipline' in education by challenging the notions, foundations, and paradigms that underpin its use in policy and practice. It confronts the understanding of 'discipline' as purely repressive, and raises the possibility of enabling forms and conceptualizations of 'discipline' that challenge tokenistic avenues for students' liberation and enhance students' capacity for agency. This book is an essential resource for university lecturers, pre-service and in-service teachers, policymakers, and educational administrators who want to re-think 'discipline' in education in ways that move beyond a concern with managing disorder, to generate alternative understandings that can make a difference in students' lives.

The second in a rip-roaring adventure series from a NEW YORK TIMES bestselling author, this is THE NEVERENDING STORY for a new generation. When the magical waters of the Pirate Stream begin flooding Marrill's world, she must return to the Stream and find the source of the mysterious Iron Tide. Reunited with her best friend Fin - who has been forgotten all over again - Marrill must make the treacherous trek to the impossible world of Monerva and uncover the secrets of its long-lost wish machine. Only there can Fin wish to finally be remembered. Only there can Marrill wish to save her world and the people she loves. But to get everything they've ever wanted, Marrill and Fin may have to give up on the most important thing they already have: each other. An epic adventure series from husband-and-wife team, John Parke Davis and Carrie Ryan. Beautifully illustrated by Todd Harris.

Small-scale fisheries make up a large proportion of world's fisheries, both by catch and participation. Effective management is essential to ensure access to fish for food and income. Covering social and economic aspects of the fishery management and governance challenge, this book provides guidance on innovative and alternative management measures and methods for small-scale fisheries. The book covers key topics such as rights, policy, co-management, communications and trade, and is an important reference for researchers and students in fisheries science and management as well as fisheries research organizations and policymakers.

* Covers all aspects of the data acquisition system from design and specification to programming, installation and configuration
* Gives both the novice and experienced user a solid understanding of interfacing the PC and standalone instruments to real-world signals from the laboratory to the industrial plant
* Provides a thorough grasp of PC data acquisition systems and the ability to design, specify, install and configure and program data acquisition systems quickly and effectively
This book focuses on data acquisition and control using the PC and standalone instruments. The PC has made a dramatic impact in the ease with which the technician, scientist and engineer today can set up their own test and measurement system at a remarkably low cost. And this book aims to show you how easy it is with plenty of carefully researched information. The popular IEEE 488 interface is also covered. All aspects of the data acquisition system are included from design and specification to programming, installation and configuration. This book gives both the novice and experienced user a solid grasp of the principles and practical implementation of interfacing the PC and standalone instruments to real-world signals from the laboratory to the industrial plant. Once you have read the book, you will have a thorough grasp of PC data acquisition systems and will be able to design, specify, install and configure and program data acquisition systems quickly and effectively.
* Covers all aspects of the data acquisition system from design and specification to programming, installation and configuration
* Gives both the novice and experienced user a solid understanding of interfacing the PC and standalone instruments to real-world signals from the laboratory to the industrial plant
* Provides a thorough grasp of PC data acquisition systems and the ability to design, specify, install and configure and program data acquisition systems quickly and effectively

Instrumentation and control systems are highly reliant on data communications, so a working knowledge of the latest communications technologies and the essential protocols is essential for anyone designing, specifying or using instrumentation and control systems. This book is the only title on the market designed specifically for this audience. This is a comprehensive treatment of industrial data communication systems. Commencing with a thorough discussion of the popular RS-232, RS-422 and RS-485 standards it then moves on to industrial protocols, industrial networks and the communication requirements for the 'smart' instrumentation which is becoming "de rigeur" in industry today. The book also provides a solid grounding in the various Fieldbus and DeviceNet standards on the market today. This book provides you with the knowledge to analyse, specify and debug data communications systems in the instrumentation and control environment.
*The essential guide to communications techologies and protocols for engineers designing, specifying or using instrumentation and control systems
*Provides the knowledge required to analyze, specify and debug data communication systems, introducing the latest digital technologies
*Coverage includes RS-232, RS422 and RS-485 standards, industrial networks and protocols, smart instrumentation, FieldBus and DeviceNet standards

Prince or pretender is a fantasy set in the kingdom of Connaught in the west of Ireland. Join Oisin and his young friend, Ellen as they search for the truth about Oisin's origins. Is it possible that Oisin is in fact a fairy Prince who was swapped at birth for a human child by an evil fairy Queen? If so, what has happened to the real Oisin Freel? Their quest for the truth takes the pair on a long and interesting journey through Ireland before ending up on the magical Hill of Tara. They met many interesting but not always helpful characters along the way. Find out if Oisin Freel is in fact a Prince or is he a pretender?

Lucy and Max must leave school early because the roads are flooded. The heavy rain washes away the bridge so there will be no school for Lucy and Max for a few days, but Lucy needs to practice playing her violin, so she will be ready for the upcoming school concert. Problem, Lucy has left her violin at school and is very upset because she knows she must practice to be good enough to be allowed to play at the concert. Her old friend, Mary comes to the rescue and lends her the violin that belonged to Mary's father. Lucy is still not sure that she will be good enough and asks for help from Mary's now departed little brother, Brian who once used to play the same violin. Will the bridge be fixed in time for the concert? Will Mrs. Wallace even let Lucy play at the concert? And if so, will the spirit of little Brian be there to help her be the best that she can be?

Lucy and her little brother, Max set out on an adventure to the abandoned school not far away from the farm their family had recently moved to. It was turning out to be a pleasant and normal adventure until they got caught in a thunder storm. It was then that the real adventure started. Lucy and Max had cope with some very strange situations. They were both sure that no one was ever going to believe when it was time for their morning talks at school on Monday morning.

Freddy spends his weekend staying a bush cabin with is father and their pet dog, Sue. They have lots of adventures hiking around the bush clad hills behind the coastal township of Tapu. Tapu is situated on the Coromandel Peninsula of New Zealand's north island. On of Freddy's adventures is to catch fish from their small boat and then to cook them fresh on the beach. Freddy's evening often end with a long soak in their wonderful bush-bath while his father tell him stories related to days activities.

Simon's young body is crippled with arthritis and he can see little hope of living a normal active life. That is until the day he has a chance encounter with, Rory of the fields. Rory is part of a band of misplaced Irish fairies that for many years have managed to remain hidden from the rest of mankind, concealed in their fortress far back in the misty hills of New Zealand's Coromandal mountain range. The fairies have been too long and too far from the source of their magic and doubt that they can help Simon but agree to try so long as Simon completes a list of tasks that the fairies set for him. First Simon must help someone with greater needs than his own, but who? His mother? No, her needs are too closely related to his own, that would not be accepted by the Fairies. But who? Simon finds the answer in the form of Shannon. The young girl who catches the school bus at the same bus stop as Simon. Together they set out to end the chaos that has become Shannan's life.