Arguably the most influential political writer of the twentieth century, George Orwell remains a crucial voice for our times. Known world-wide for his two best-selling masterpieces Nineteen Eighty-Four, a gripping portrait of a dystopian future, and Animal Farm, a brilliant satire on the Russian Revolution, Orwell has been revered as an essayist, journalist and literary-political intellectual, and his works have exerted a powerful international impact on the post-World War Two era. This Introduction examines Orwell's life, work and legacy, addressing his towering achievement and his ongoing appeal. Combining important biographical detail with close analysis of his writings, the book considers the various genres in which Orwell wrote: the realistic novel, the essay, journalism and the anti-utopia. Ideally suited for readers approaching Orwell's work for the first time, the book concludes with an extended reflection on why George Orwell has enjoyed a literary afterlife unprecedented among modern authors in any language.

Arguably the most influential political writer of the twentieth century, George Orwell remains a crucial voice for our times. Known world-wide for his two best-selling masterpieces Nineteen Eighty-Four, a gripping portrait of a dystopian future, and Animal Farm, a brilliant satire on the Russian Revolution, Orwell has been revered as an essayist, journalist and literary-political intellectual, and his works have exerted a powerful international impact on the post-World War Two era. This Introduction examines Orwell's life, work and legacy, addressing his towering achievement and his ongoing appeal. Combining important biographical detail with close analysis of his writings, the book considers the various genres in which Orwell wrote: the realistic novel, the essay, journalism and the anti-utopia. Ideally suited for readers approaching Orwell's work for the first time, the book concludes with an extended reflection on why George Orwell has enjoyed a literary afterlife unprecedented among modern authors in any language.

Understanding gene function and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols cover a variety of methods, both well established and newly evolving. These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. The introductory matter to each chapter includes concise technical and theoretical discussions with considerations for selection of the appropriate system and strategies for delivery.