Understanding gene function and regulation requires rigorous
testing in live cells and organisms. Recent advances have provided
a variety of new strategies for delivering DNA and RNA into cells
and probing their expression, as well as new clinical applications
that rely upon the introduction of genetic material. The vast
number of available techniques for clinical and laboratory research
often makes selecting the optimal method a difficult process. Gene
Transfer: Delivery and Expression of DNA and RNA provides the first
comprehensive guide to technical approaches for delivering nucleic
acids into cells and organisms and of ensuring (even manipulating)
appropriate expression. The detailed, step-by-step protocols cover
a variety of methods, both well established and newly evolving.
These include viral and nonviral methods of gene delivery,
transgenic approaches, strategies for the regulation of transgene
expression, and modification of the host response. The introductory
matter to each chapter includes concise technical and theoretical
discussions with considerations for selection of the appropriate
system and strategies for delivery.
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