Spanning her entire life, the fully annotated selections in this volume include well known recollections of the great Victorian novelist plus a large assortment not found in her biographies. Altogether they provide a fresh, vivid, and sometimes startling portrait of a controversial genius.

Recombinant viruses provide an efficient mechanism for the transfer and expression of DNA in eukaryotic cells. First, the transfer of DNA by viral infection-utilizing specific cell surface receptors and cellular intern- ization mechanisms-occurs much more readily than DNA transfer via uptake induced by such physical methods as calcium phosphate coprecipitation or electroporation. Second, the novel strategies employed by the virus to express its own genes can then be "hijacked" in the recombinant virus to express the researcher's gene of interest The purpose of Practical Molecular Virology isthus to compile a coll- tion of readily repeatable gene transfer and expression methods from wo- ers expert in the use of a variety of recombinant viral vectors . These include those designed for the production of recombinant antigens, such as pol- virus and yeast Ty-VLPs; those giving very high levels of recombinant protein expression, for example, baculovirus, vaccinia virus, and SV40; and finally viral vectors used for efficient, stable gene transfer to eu otic cells, such as retroviruses and herpesviruses . The first chapter describes the viral life cycle for each virus, and explains how this can be adapted to allow construction of recombinant vectors. Subsequent chapters deal with methods for producing and char- terizing recombinant viruses . I make no apology for the hyperproliferation of chapters dealing with recombinant retroviral methods and applications, since I believe this is clearly proportional to the recent expansion of interest in these techniques.

"31 Months in Japan--the Building of a Theme Park" is the story of two Southern Californians embarking on the adventure of a lifetime--moving to Japan to participate in the construction of the Universal Studios Japan(R) theme park.

Join authors Larry K. & Lorna Collins on an odyssey into a foreign culture where they are often misunderstood and frequently confused. Discover the challenges and frustrations of building a world-class entertainment venue outside the United States. Enjoy the adventure as they discover the beauty and the people of Japan. Share their laughter, tears, surprise and final triumph as the park is completed ahead of schedule and under budget. Meet fascinating characters like Jurassic Jack, the Nihon Cowboy and Dote-san through their eyes.

"31 Months in Japan--the Building of a Theme Park" combines interesting details and behind-the-scenes descriptions of how magic is made in the entertainment industry with funny and touching personal remembrances. The stories will be of particular interest to those visiting or intending to live or work in Japan, people doing business with the Japanese, theme park aficionados and anyone with a sense of adventure.

This book provides a legal historical insight into colonial laws on enslavement and the plantation system in the British West Indies. The volume is a work of comparative legal history of the English-speaking Caribbean which concentrates on how the laws of England served to catalyse the slavery laws and also legislation pertaining to post-emancipation societies. The book illustrates how these “borrowed� laws from England not only developed colonial slavery laws within the English-speaking Caribbean but also inspired the slavery codes of a number of North American plantation systems. The cusp of the work focuses on the interconnectivities among the English-speaking slave holding Atlantic and how persons, free and unfree, moved throughout the system and brought laws with them which greatly affected the various enslaved societies. The book will be essential reading for students and researchers interested in colonial slavery, Caribbean studies and Black and Atlantic history.

This book provides a legal historical insight into colonial laws on enslavement and the plantation system in the British West Indies. The volume is a work of comparative legal history of the English-speaking Caribbean which concentrates on how the laws of England served to catalyse the slavery laws and also legislation pertaining to post-emancipation societies. The book illustrates how these "borrowed" laws from England not only developed colonial slavery laws within the English-speaking Caribbean but also inspired the slavery codes of a number of North American plantation systems. The cusp of the work focuses on the interconnectivities among the English-speaking slave holding Atlantic and how persons, free and unfree, moved throughout the system and brought laws with them which greatly affected the various enslaved societies. The book will be essential reading for students and researchers interested in colonial slavery, Caribbean studies and Black and Atlantic history.

Spanning her entire life, the fully annotated selections in this volume include well known recollections of the great Victorian novelist plus a large assortment not found in her biographies. Altogether they provide a fresh, vivid, and sometimes startling portrait of a controversial genius.

Artificial Reefs in European Seas focuses on artificial reef research in the Mediterranean and NE Atlantic. The book describes most of the long-term projects running in European seas, presents the legal and economic issues, and suggests future uses for artificial reefs in the European context. Readership: Professionals working on or interested in the uses of artificial reefs for fishery management, coastal zone management, aquaculture and nature conservation. The case studies of reef research programmes make the book ideal for degree students studying topics in ecology, and fisheries and coastal management.

Artificial Reefs in European Seas focuses on artificial reef research in the Mediterranean and NE Atlantic. The book describes most of the long-term projects running in European seas, presents the legal and economic issues, and suggests future uses for artificial reefs in the European context. Readership: Professionals working on or interested in the uses of artificial reefs for fishery management, coastal zone management, aquaculture and nature conservation. The case studies of reef research programmes make the book ideal for degree students studying topics in ecology, and fisheries and coastal management.

Recombinant viruses provide an efficient mechanism for the transfer and expression of DNA in eukaryotic cells. First, the transfer of DNA by viral infection-utilizing specific cell surface receptors and cellular intern- ization mechanisms-occurs much more readily than DNA transfer via uptake induced by such physical methods as calcium phosphate coprecipitation or electroporation. Second, the novel strategies employed by the virus to express its own genes can then be "hijacked" in the recombinant virus to express the researcher's gene of interest The purpose of Practical Molecular Virology isthus to compile a coll- tion of readily repeatable gene transfer and expression methods from wo- ers expert in the use of a variety of recombinant viral vectors . These include those designed for the production of recombinant antigens, such as pol- virus and yeast Ty-VLPs; those giving very high levels of recombinant protein expression, for example, baculovirus, vaccinia virus, and SV40; and finally viral vectors used for efficient, stable gene transfer to eu otic cells, such as retroviruses and herpesviruses . The first chapter describes the viral life cycle for each virus, and explains how this can be adapted to allow construction of recombinant vectors. Subsequent chapters deal with methods for producing and char- terizing recombinant viruses . I make no apology for the hyperproliferation of chapters dealing with recombinant retroviral methods and applications, since I believe this is clearly proportional to the recent expansion of interest in these techniques.

Warrior Mother is the true story of a mother's fierce love and determination, and her willingness to go outside the bounds of the ordinary when two of her three adult children are diagnosed with life-threatening diseases.

When Sheila Collins's best friend, dying of breast cancer, asked her to accompany her through what turned out to be the last fourteen days of her life, she didn't know that the experience was preparing her for what lay ahead with her own children.

In the years that followed, Collins had to face both her son's diagnosis with AIDS and her daughter's diagnosis with breast cancer. Warrior Mother documents how she faces these challenges and the issues accompanying them from learning to be the mother of a gay son to visiting a healer in Brazil on her daughter's behalf when she decides on bone marrow transplant treatment. Experience as a professional social worker and family therapist doesn't always help Collins to cope with her children's illnesses...