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Books > Science & Mathematics > Biology, life sciences > Life sciences: general issues > Genetics (non-medical)
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Practical Molecular Virology (Paperback, 1991 ed.)
Loot Price: R3,004
Discovery Miles 30 040
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Practical Molecular Virology (Paperback, 1991 ed.)
Series: Methods in Molecular Biology, 8
Expected to ship within 10 - 15 working days
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Recombinant viruses provide an efficient mechanism for the transfer
and expression of DNA in eukaryotic cells. First, the transfer of
DNA by viral infection-utilizing specific cell surface receptors
and cellular intern- ization mechanisms-occurs much more readily
than DNA transfer via uptake induced by such physical methods as
calcium phosphate coprecipitation or electroporation. Second, the
novel strategies employed by the virus to express its own genes can
then be "hijacked" in the recombinant virus to express the
researcher's gene of interest The purpose of Practical Molecular
Virology isthus to compile a coll- tion of readily repeatable gene
transfer and expression methods from wo- ers expert in the use of a
variety of recombinant viral vectors . These include those designed
for the production of recombinant antigens, such as pol- virus and
yeast Ty-VLPs; those giving very high levels of recombinant protein
expression, for example, baculovirus, vaccinia virus, and SV40; and
finally viral vectors used for efficient, stable gene transfer to
eu otic cells, such as retroviruses and herpesviruses . The first
chapter describes the viral life cycle for each virus, and explains
how this can be adapted to allow construction of recombinant
vectors. Subsequent chapters deal with methods for producing and
char- terizing recombinant viruses . I make no apology for the
hyperproliferation of chapters dealing with recombinant retroviral
methods and applications, since I believe this is clearly
proportional to the recent expansion of interest in these
techniques.
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