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Agrobacterium Protocols - Volume 1 (Hardcover, 3rd ed. 2015): Kan Wang Agrobacterium Protocols - Volume 1 (Hardcover, 3rd ed. 2015)
Kan Wang
R5,513 R5,052 Discovery Miles 50 520 Save R461 (8%) Ships in 12 - 17 working days

Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included. Volume 1 details updated techniques available for 18 plant species drawn from cereal crops, legume plants, vegetable plants, and three model plant species: Brachypodium distachyon, Medicago truncatula, and Setaria viridis. It also updates a chapter for vector construction, a step critical to a successful plant transformation process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers for use in both fundamental and applied biology.

Agrobacterium Protocols - Volume 2 (Hardcover, 3rd ed. 2015): Kan Wang Agrobacterium Protocols - Volume 2 (Hardcover, 3rd ed. 2015)
Kan Wang
R4,968 R3,757 Discovery Miles 37 570 Save R1,211 (24%) Ships in 12 - 17 working days

Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included. Volume 2 contains 29 chapters with updated techniques for industrial plants, root plants, nuts and fruits, tropic plants, and other important plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers in both fundamental and applied biology.

Agrobacterium Protocols - Volume II (Hardcover, 2nd ed. 2007): Kan Wang Agrobacterium Protocols - Volume II (Hardcover, 2nd ed. 2007)
Kan Wang
R4,340 Discovery Miles 43 400 Ships in 10 - 15 working days

Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric- tural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger "gene silencing" often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Ag- bacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent devel- ments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

Agrobacterium Protocols - Volume I (Hardcover, 2nd ed. 2006): Kan Wang Agrobacterium Protocols - Volume I (Hardcover, 2nd ed. 2006)
Kan Wang
R5,538 Discovery Miles 55 380 Ships in 10 - 15 working days

Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agricultural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate a single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger "gene silencing" often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Agrobacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent developments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

Protoplast Technology - Methods and Protocols (Hardcover, 1st ed. 2022): Kan Wang, Feng Zhang Protoplast Technology - Methods and Protocols (Hardcover, 1st ed. 2022)
Kan Wang, Feng Zhang
R6,273 R5,369 Discovery Miles 53 690 Save R904 (14%) Ships in 12 - 17 working days

This detailed volume collects protocols from scientists who are actively engaged in developing or using protoplast technology. The book begins with chapters that focus on basic protoplast techniques and their utilities, such as protocols on protoplast isolation, transfection, and regeneration, as well as examples of how to use protoplasts for genome editing and gene function analysis in a number of major crop or model plant species. It continues by exploring protoplast automation, large scale functional genomics, and synthetic biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protoplast Technology: Methods and Protocols aims to inspire the new generation of researchers to further improve their protocols and apply this technology to accelerate the field of plant genomic study.

Agrobacterium Protocols - Volume 2 (Paperback, Softcover reprint of the original 3rd ed. 2015): Kan Wang Agrobacterium Protocols - Volume 2 (Paperback, Softcover reprint of the original 3rd ed. 2015)
Kan Wang
R4,852 Discovery Miles 48 520 Ships in 10 - 15 working days

Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included. Volume 2 contains 29 chapters with updated techniques for industrial plants, root plants, nuts and fruits, tropic plants, and other important plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers in both fundamental and applied biology.

Agrobacterium Protocols - Volume 1 (Paperback, Softcover reprint of the original 3rd ed. 2015): Kan Wang Agrobacterium Protocols - Volume 1 (Paperback, Softcover reprint of the original 3rd ed. 2015)
Kan Wang
R4,353 Discovery Miles 43 530 Ships in 10 - 15 working days

Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included. Volume 1 details updated techniques available for 18 plant species drawn from cereal crops, legume plants, vegetable plants, and three model plant species: Brachypodium distachyon, Medicago truncatula, and Setaria viridis. It also updates a chapter for vector construction, a step critical to a successful plant transformation process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers for use in both fundamental and applied biology.

Agrobacterium Protocols - Volume II (Paperback, Softcover reprint of hardcover 2nd ed. 2007): Kan Wang Agrobacterium Protocols - Volume II (Paperback, Softcover reprint of hardcover 2nd ed. 2007)
Kan Wang
R4,285 Discovery Miles 42 850 Ships in 10 - 15 working days

Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric- tural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger "gene silencing" often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Ag- bacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent devel- ments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

Agrobacterium Protocols - Volume I (Paperback, Softcover reprint of hardcover 2nd ed. 2006): Kan Wang Agrobacterium Protocols - Volume I (Paperback, Softcover reprint of hardcover 2nd ed. 2006)
Kan Wang
R5,484 Discovery Miles 54 840 Ships in 10 - 15 working days

Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agricultural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate a single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger "gene silencing" often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Agrobacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent developments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

Transformation of Plants and Soil Microorganisms (Paperback, Revised): Kan Wang, Alfredo Herrera-Estrella, Marc Van Montagu Transformation of Plants and Soil Microorganisms (Paperback, Revised)
Kan Wang, Alfredo Herrera-Estrella, Marc Van Montagu
R1,381 Discovery Miles 13 810 Ships in 12 - 17 working days

Over the past fifty years plant breeders have achieved impressive improvements in yield, quality and disease resistance. These gains suggest that many more modifications might be introduced if appropriate genes can be identified. Current DNA techniques allow the construction of transgenic plants and this important new book reviews the current state of knowledge. A team of leading researchers provide in-depth reviews at the cutting edge of technology for laboratory techniques for the transformation of important soil microorganisms and recalcitrant plants of economic value. The book is divided into three sections: soil microorganisms; cereal crops; and industrially important plants. The most effective methods used to date are compared, and their merits and limitations discussed. Some chapters emphasise case studies and applications. In cases where obstacles remain to be overcome, an overview of progress to date is given. The book will serve as a general guide and reference tool for those working on transformation in microbiology and plant science.

Protoplast Technology - Methods and Protocols (1st ed. 2022): Kan Wang, Feng Zhang Protoplast Technology - Methods and Protocols (1st ed. 2022)
Kan Wang, Feng Zhang
R3,523 Discovery Miles 35 230 Ships in 10 - 15 working days

This detailed volume collects protocols from scientists who are actively engaged in developing or using protoplast technology. The book begins with chapters that focus on basic protoplast techniques and their utilities, such as protocols on protoplast isolation, transfection, and regeneration, as well as examples of how to use protoplasts for genome editing and gene function analysis in a number of major crop or model plant species. It continues by exploring protoplast automation, large scale functional genomics, and synthetic biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Protoplast Technology: Methods and Protocols aims to inspire the new generation of researchers to further improve their protocols and apply this technology to accelerate the field of plant genomic study.

People, Process, and Technology Management Framework - A Manager's Practical Guide to Establishing a Structure for Growth... People, Process, and Technology Management Framework - A Manager's Practical Guide to Establishing a Structure for Growth (Paperback)
Kan Wang
R167 Discovery Miles 1 670 Ships in 10 - 15 working days

Kyle, the IT manager in the company, found he spent most of his time trying to explain what information technology could and couldn't do as he created a patchwork of applications to solve individual problems. As soon as upper management heard that Matt, the operational manager, had found a way to shave a day or two off of order fulfillment with a new application idea, Kyle knew they would tell him to drop everything and make it happen.

However, Kyle needed answers to a list of questions (requirements) before he could help. Unfortunately, despite his competence, Matt couldn't answer the majority of his questions, and had absolutely no clue when it came to IT. He was angry that Kyle made it seem like a monumental task to take a simple Microsoft Word document that the sales department already had, and just speed up how soon his production planner received that information via an application to pull it from sales. At the conclusion of their meeting, both parties walked out feeling they had accomplished nothing. Creating yet another specialized application would ultimately generate future problems for both Kyle and Matt's departments, and yet these two well-intentioned managers were about to spend thousands of dollars in application development, man-hours, training, and productivity.

The problem was, they were speaking different languages. If Kan had been present at this hypothetical meeting, "I could have interpreted between the two functional areas," he says, "but only because of my background in operations and IT management-a background most people on either side do not have. I have been on both sides of the table, as an IT manager like Kyle, administering IT projects and resources, and as an operations manager like Matt, helping businesses reach the next level of operational effectiveness. I have worked with fledgling start-ups and with companies employing thousands of people, in industries as diverse as e-commerce, manufacturing, and government. My career spans from an-in-the-trenches IT developer at the bottom to a senior manager at the top of it. Even my education encompasses both business and IT with a master's in computer science and an MBA."

Coming across hundreds of scenarios similar to Matt and Kyle's led Wang to design the People, Process, and Technology Management Framework to help companies deal with the immediate, secondary, and/or long-term problems the lack of efficient communication between departments caused. The PPT Framework was not developed to answer all of a company's problems-nor was it meant to capture every single piece of data and information within a company's operations. Rather, it is a powerful methodology that provides a stable platform for managing growth and complexity, affording stability while also being flexible enough to allow for rapid operational change. Similarly, the book's purpose is not to provide a step-by-step guide for implementing the PPT Framework for a specific industry, company, department, or process. It is a broad reference for those considering the need for the framework as well as those who are in the process of implementing it.

"Implementing the PPT Framework never truly ends," says the author. "As a business expands, as the market changes, as people come and go, and as new technologies are incorporated, a company continuously changes. Thus, this book addresses the specific elements of the framework-the building blocks, if you will-and provides an illustration of how it could be implemented by using Matt and Kyle's situation as an example." Due to its scalability, the PPT Framework is just as applicable to small startup businesses as it is to companies that employ thousands of people, and one of its appealing aspects lies in its simplicity. After turning the last page of this inspiring, enlightening, and highly unique guide, it's guaranteed readers will be motivated to say, "I can do this It will help my career as a manager."

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