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Books > Science & Mathematics > Biology, life sciences > Cellular biology
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Agrobacterium Protocols - Volume I (Hardcover, 2nd ed. 2006)
Loot Price: R5,538
Discovery Miles 55 380
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Agrobacterium Protocols - Volume I (Hardcover, 2nd ed. 2006)
Series: Methods in Molecular Biology, 343
Expected to ship within 10 - 15 working days
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Total price: R5,558
Discovery Miles: 55 580
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Agrobacterium tumefaciens is a soil bacterium that for more than a
century has been known as a pathogen causing the plant crown gall
disease. Unlike many other pathogens, Agrobacterium has the ability
to deliver DNA to plant cells and permanently alter the plant
genome. The discovery of this unique feature 30 years ago has
provided plant scientists with a powerful tool to genetically
transform plants for both basic research purposes and for
agricultural development. Compared to physical transformation
methods such as particle bomba- ment or electroporation,
Agrobacterium-mediated DNA delivery has a number of advantages. One
of the features is its propensity to generate a single or a low
copy number of integrated transgenes with defined ends. Integration
of a single transgene copy into the plant genome is less likely to
trigger "gene silencing" often associated with multiple gene
insertions. When the first edition of Agrobacterium Protocols was
published in 1995, only a handful of plants could be routinely
transformed using Agrobacterium. Agrobacterium-mediated
transformation is now commonly used to introduce DNA into many
plant species, including monocotyledon crop species that were
previously considered non-hosts for Agrobacterium. Most remarkable
are recent developments indicating that Agrobacterium can also be
used to deliver DNA to non-plant species including bacteria, fungi,
and even mammalian cells.
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