Keith Morris is a true punk icon. No one else embodies the sound of Southern Californian hardcore the way he does. With his waist-length dreadlocks and snarling vocals, Morris is known the world over for his take-no-prisoners approach on the stage and his integrity off of it. Over the course of his forty-year career with Black Flag, the Circle Jerks, and OFF!, he's battled diabetes, drug and alcohol addiction, and the record industry...and he's still going strong. My Damage is more than a book about the highs and lows of a punk rock legend. It's a story from the perspective of someone who has shared the stage with just about every major figure in the music industry and has appeared in cult films like The Decline of Western Civilization and Repo Man. A true Hollywood tale from an L.A. native, My Damage reveals the story of Morris's streets, his scene, and his music--as only he can tell it.

Fluorescence in situ Hybridization (FISH) belongs to that special category of well-established molecular biology techniques that, since their inception a few decades ago, have succeeded in keeping a prominent position within the constantly expanding list of laboratory pro- dures for biomedical research and clinical diagnostics. The design simplicity and cost-effectiveness of the early FISH protocols, combined with the signifcant acceleration of discoveries in related technical areas such as fuor- cence microscopy, digital imaging, and nucleic acid technology have prompted the div- sifcation of the original technique into an outstanding number of imaginative and useful applications, and thus have not only held back its outmoding but have also promoted its expansion into different areas of basic and applied research in the post-genomic era. The 34 chapters included in this book aim at portraying the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of va- ous applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology, and evolutionary st- ies. The book is divided in four parts: (I) Core Techniques, (II) Technical Advancements and Novel Adaptations, (III) Translational FISH: Applications for Human Genetics and Medicine, and (IV) Protocols for Model Organisms.

Fluorescence in situ Hybridization (FISH) belongs to that special category of well-established molecular biology techniques that, since their inception a few decades ago, have succeeded in keeping a prominent position within the constantly expanding list of laboratory pro- dures for biomedical research and clinical diagnostics. The design simplicity and cost-effectiveness of the early FISH protocols, combined with the signifcant acceleration of discoveries in related technical areas such as fuor- cence microscopy, digital imaging, and nucleic acid technology have prompted the div- sifcation of the original technique into an outstanding number of imaginative and useful applications, and thus have not only held back its outmoding but have also promoted its expansion into different areas of basic and applied research in the post-genomic era. The 34 chapters included in this book aim at portraying the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of va- ous applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology, and evolutionary st- ies. The book is divided in four parts: (I) Core Techniques, (II) Technical Advancements and Novel Adaptations, (III) Translational FISH: Applications for Human Genetics and Medicine, and (IV) Protocols for Model Organisms.

Microscopy, which has served as a fundamental scientific technique for centuries, remains an invaluable tool in chemistry, biology, healthcare, and forensics. Increasingly, it is being integrated into modern chemical instrumentation and is of value as a powerful analytical tool across many scientific disciplines.

Designed to serve as a primary resource for undergraduate or graduate students, An Introduction to Microscopy helps students master the foundational principles of microscopy. Intentionally concise, this text does not attempt to cover all aspects of all types of microscopy such as polarizing light and fluorescence. Instead, the authors' intent is to provide students with the basic knowledge necessary to explore and understand these more advanced techniques. The authors draw from their own extensive backgrounds in forensic identification to explain the methods and ways in which microscopy shapes every investigation.

All nine chapters include questions and most include simple exercises related to the material covered. Numerous figures and photographs supplement the text and explain the procedures and principles introduced. A glossary is included as well as a convenient list of abbreviations, and references to more in-depth readings.

Offers a Fundamental Approach for Students in all Fields

The material assumes basic mathematics skill through algebra and a basic knowledge of fundamental chemistry and physics (essential for understanding optics). Although the authors used the high-quality microscopes found in their laboratories to produce the images found in the book, the information and methods can be applied to any type of microscope to which students have access.

Understanding the fundamentals of microscopy provides students with a relevant and marketable skill that can be readily applied in many fields, even if the students have not had significant academic training in the subject. Furthermore, by understanding various aspects of microscopy, students will begin to understand the science behind other related areas, such as spectroscopy, optics, and any number of applications involving analytical instrumentation.