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Ex situ preservation of germplasm for higher plant species has been
accom plished using either seeds or clones, but storage of these
under typical condi tions does not provide the extreme longevities
that are needed to minimize risk of loss. Costs of maintenance and
regeneration of stocks are also high. Systems that provide
virtually indefinite storage should supplement existing methods and
it is within this context that cryopreservation is presented. The
use of low temperature preservation was initially more a concern of
medicine and animal breeding, and was expanded to plants in the
1970s. Sur vival after cryogenic exposure has now been demonstrated
for diverse plant groups including algae, bryophytes, fungi and
higher plants. If survival is com monplace, then the eventual
application is a cryopreservation system, whereby cells, tissues
and organs are held indefinitely for use, often in the unforeseen
future. The increasing interest and capabilities for application
could not have occurred at a more opportune time since expanding
human populations have placed unprecedented pressures on plant
diversity. This book emphasizes cry opreservation of higher plants
and was initially driven by the concern for loss of diversity in
crops and the recognized need that this diversity would be
essential for continued improvement of the many plants used by
society for food, health and shelter. The interest in
cryopreservation has been expanded by conservationists and their
concerns for retaining, as much as possible, the diversity of
natural populations. The need for cryopreservation, thus, is well
established."
This volume highlights achievements in cryopreservation, chronicles method development, and describes relevant literature. The provided detailed information helps practitioners develop and improve methods for desired species. The volume is divided into four parts:I. Cryopreservation of Germplasm;II. Herbaceous Plants: Barley, celery, chamomile, chicory, garlic, ginseng, hop, horseradish, mint, taro, wasabi;III. Woody Species: Coffee, Eucalyptus, guazuma, horse-chestnut, neem, olive, poplar, oak, Prunus, Ribes, rose.IV. Australian Species.Initially, cryopreservation was driven by the concern for loss of diversity of crops essential for continued improvement of the many plants used for food, health, and shelter. The interest has been expanded by conservationists and their concerns for retaining the diversity of natural populations.
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