The introduction of femtosecond pulse lasers has provided numerous new methods for non-destructive diagnostic analysis of biological samples. This book is the first to provide a focused and systematic treatment of femtosecond biophotonic methods. Each chapter combines theory, practice and applications, walking the reader through imaging, manipulation and fabrication techniques. Beginning with an explanation of nonlinear and multiphoton microscopy, subsequent chapters address the techniques for optical trapping and the development of laser tweezers. In a conclusion that brings together the various topics of the book, the authors discuss the growing field of femtosecond micro-engineering. The wide range of applications for femtosecond biophotonics means this book will appeal to researchers and practitioners in the fields of biomedical engineering, biophysics, life sciences and medicine.