This volume describes protocols for basic state-of-the-art approaches in the field of peptidomics. Most of these approaches are independent of the instruments used for analysis and can easily be adapted for equipment that is available in a typical proteomics facility. Chapters detail many of the basic techniques used to detect and identify peptides, methods for the relative quantitation of peptides between samples using isotopic labels or label-free approaches, and biological species as well as sample types. Written in the highly successful format of the Methods in Molecular Biology series, each chapter includes an introduction to the topic, a list of the necessary materials and reagents, reproducible step-by-step laboratory protocols, and tips on troubleshooting common problems and avoiding pitfalls. Authoritative and practical, Peptidomics: Methods and Strategies provides useful guidance for studies in the rapidly growing field of peptidomics.

Many people think that they do not need a book on "how to write a really bad grant application" - they already know how. Actually, this book is intended as a guide to writing grant applications that will get funded. One way to write a good grant application is to avoid writing a bad one. This book analyzes "bad" scientific grant applications from a humorous perspective. There are also some sections on obtaining funding from private foundations and industry, and a few parts that are pure silliness. All pieces in this book are original and are either fiction (the humor pieces) or non-fiction (the serious advice). The reader will hopefully be able to tell the two apart.