This book brings together a unique collection of protocols that cover novel and specialized techniques as well as updated and improved adaptations of more standard procedures involving the cell cycle and its regulation by oscillatory networks, exploring recent progress in the field from both holistic and reductionist perspectives. The edition provides a space for researchers to highlight and explore the latest developments in molecular biology and biochemical techniques for studying oscillatory networks and to share these across the research community to facilitate further progress. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Cell Cycle Oscillators: Methods and Protocols, Second Edition serves as an invaluable guide for those new to the field as well as the more experienced scientist. Chapters 19 and 21 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This book brings together a unique collection of protocols that cover novel and specialized techniques as well as updated and improved adaptations of more standard procedures involving the cell cycle and its regulation by oscillatory networks, exploring recent progress in the field from both holistic and reductionist perspectives. The edition provides a space for researchers to highlight and explore the latest developments in molecular biology and biochemical techniques for studying oscillatory networks and to share these across the research community to facilitate further progress. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Cell Cycle Oscillators: Methods and Protocols, Second Edition serves as an invaluable guide for those new to the field as well as the more experienced scientist. Chapters 19 and 21 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Communication and intimacy can feel like a constant struggle in relationships where one partner has Asperger Syndrome (AS). For the neuro-typical partner (NT) in particular, this can be an endless source of frustration, misunderstandings, and tears. Drawing on her own experience of being married to a man with AS, Louise Weston shows that the road to intimacy begins with letting go of expectations and looking after your own physical and emotional needs. She provides tried-and-tested strategies for relating to and connecting with your AS partner, as well as useful tips for coping with hurtful words and meltdowns, helping your partner to interpret emotions, and finding further sources of help and support. Above all, she shows that although your AS/NT relationship will challenge you beyond what you ever thought possible, by letting go of expectations and respecting each others' differences, this unique partnership really can be both happy and successful. Brimming with stories and advice from other NT partners, this practical book will help NTs take positive steps towards connecting with their AS partners. It will also be a useful resource for counsellors and other professionals who wish to deepen their understanding of AS/NT relationships.

This volume brings together a unique collection of protocols that cover standard, novel, and specialized techniques. Cell Cycle Oscillators: Methods and Protocols guides readers through recent progress in the field from both holistic and reductionist perspectives, providing the latest developments in molecular biology techniques, biochemistry, and computational analysis used for studying oscillatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Cell Cycle Oscillators: Methods and Protocols will serve as an invaluable reference to gain further insight into the complex and incompletely understood processes that are involved in the cell cycle and its regulation by oscillatory networks.