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1. Surface Plasmon Resonance: A General Introduction Nico J. de Mol
and Marcel J.E. Fischer 2. The Role of Mass Transport Limitation
and Surface Heterogeneity in the Biophysical Characterization of
Macromolecular Binding Processes by SPR Biosensing Peter Schuck and
Huaying Zhao 3. Amine Coupling Through EDC/NHS: A Practical
Approach Marcel J.E. Fischer 4. High Affinity Immobilization of
Proteins Using Biotin- and GST-based Coupling Strategies Stephanie
Q. Hutsell, Randall J. Kimple, David P. Siderovski, Francis S.
Willard, and Adam J. Kimple 5. A Capture Coupling Method for the
Covalent Immobilization of Hexahistidine Tagged Proteins for
Surface Plasmon Resonance Adam J. Kimple, Robin E. Muller, David P.
Siderovski, and Francis S. Willard 6. Affinity Constants for Small
Molecules from SPR Competition Experiments Nico J. de Mol 7.
Surface Plasmon Resonance Signal Enhancement for Immunoassay of
Small Molecules John S. Mitchell and Yinqiu Wu 8. High-Throughput
Kinase Assay Based on Surface Plasmon Resonance Hiroyuki Takeda,
Naoki Goshima, and Nobuo Nomura 9. SPR Biosensor as a Tool for
Screening Prion Protein Binders as Potential Antiprion Leads
Beining Chen 10. Carbohydrate-Lectin Interactions Assayed by SPR
Eric Duverger, Nathalie Lamerant-Fayel, Natacha Frison, and Michel
Monsigny 11. DNA Sensors Based on Mixed Self-Assembled
DNA/Alkanethiol Films Sara Peeters and Tim Stakenborg 12.
Preparation of Lipid Membrane Surfaces for Molecular Interaction
Studies by Surface Plasmon Resonance Biosensors Mojca Podlesnik
Besenicar and Gregor Anderluh 13. Capture of Intact Liposomes on
Biacore Sensor Chips for Protein-Membrane Interaction Studies Vesna
Hodnik and Gregor Anderluh 14. Surface Plasmon Resonance
Spectroscopy for Studying the Membrane Binding of Antimicrobial
Peptides Kristopher Hall and Marie-Isabel Aguilar 15. Surface
Plasmon Resonance Spectroscopy in Determination of the Interactions
between Amyloid ss Proteins (Ass) and Lipid Membranes Xu Hou, David
H. Small, and Marie-Isabel Aguilar 16. Incorporation of a
Transmembrane Protein into a Supported 3D-Matrix of Liposomes for
SPR Studies Annette Graneli 17. Application of Surface Plasmon
Resonance Spectroscopy to Study G-Protein Coupled Receptor
Signalling Konstantin E. Komolov and Karl-Wilhelm Koch 18.
Integration of SPR Biosensors with Mass Spectrometry (SPR-MS)
Dobrin Nedelkov 19. SPR/MS: Recovery from Sensorchips for Protein
Identification by MALDI-TOF Mass Spectrometry Jonas Borch and Peter
Roepstorff
Surface plasmon resonance (SPR) has evolved into an exciting
technique in biomolecular interaction analysis. The development of
commercial SPR instruments has made the te- nique available to a
wide scienti?c audience, and the number of publications in which
the use of SPR is described is rapidly increasing. SPR is in use
for many purposes from food quality control to the study of
nanoparticles. Much research is now focused on devel- ing new
SPR-related applications, e.g., SPR imaging, SPR arrays, SPR
?uorescence, and combinations of SPR with mass spectrometry and
with electrochemistry. Biomolecular interaction analysis is at the
core of many research projects. In principle, the setup of an SPR
experiment is simple: There is a sensor surface to which one of the
interacting partners (the ligand) is immobilized; the other partner
(the analyte) is added in a ?ow or cell-like compartment. The
binding phenomenon is monitored in real time as a change in SPR
angle. An important issue is the choice of surface and the
immobilization strategy. With SPR, it is possible to mimic the
biological environment which is relevant for an interaction. For
interactions in a water environment, sensor surfaces with hydrogels
are available. Many biomolecular interactions take place in a
membrane environment. For this, commercial sensor surfaces are
available, or surfaces can be tailor-made. This volume contains
several examples of building up of lipophilic surfaces. Nature
abundantly makes use of multivalent interactions; multivalency can
be mimicked on a sensor surface with immobilized ligands.
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