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Books > Science & Mathematics > Biology, life sciences > Biochemistry
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Surface Plasmon Resonance - Methods and Protocols (Hardcover, Edition.)
Loot Price: R5,159
Discovery Miles 51 590
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Surface Plasmon Resonance - Methods and Protocols (Hardcover, Edition.)
Series: Methods in Molecular Biology, 627
Expected to ship within 10 - 15 working days
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Surface plasmon resonance (SPR) has evolved into an exciting
technique in biomolecular interaction analysis. The development of
commercial SPR instruments has made the te- nique available to a
wide scienti?c audience, and the number of publications in which
the use of SPR is described is rapidly increasing. SPR is in use
for many purposes from food quality control to the study of
nanoparticles. Much research is now focused on devel- ing new
SPR-related applications, e.g., SPR imaging, SPR arrays, SPR
?uorescence, and combinations of SPR with mass spectrometry and
with electrochemistry. Biomolecular interaction analysis is at the
core of many research projects. In principle, the setup of an SPR
experiment is simple: There is a sensor surface to which one of the
interacting partners (the ligand) is immobilized; the other partner
(the analyte) is added in a ?ow or cell-like compartment. The
binding phenomenon is monitored in real time as a change in SPR
angle. An important issue is the choice of surface and the
immobilization strategy. With SPR, it is possible to mimic the
biological environment which is relevant for an interaction. For
interactions in a water environment, sensor surfaces with hydrogels
are available. Many biomolecular interactions take place in a
membrane environment. For this, commercial sensor surfaces are
available, or surfaces can be tailor-made. This volume contains
several examples of building up of lipophilic surfaces. Nature
abundantly makes use of multivalent interactions; multivalency can
be mimicked on a sensor surface with immobilized ligands.
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