This volume is a collection of detailed protocols describing state-of-art approaches that will facilitate the understanding of protein homeostasis in plant stress responses and development. Plant Proteostasis: Methods and Protocols is broken into four parts focusing on the study of ubiquitin-dependent post-translational modifications, protocols focused on Ubl post-translational modifications, protein homeostasis, and protocols for the in silico analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Proteostasis: Methods and Protocols aims to address next challenges in agriculture such as precision horticulture.

This second edition compiles state-of-art protocols to study the molecular mechanisms that determine proteome composition and contribute to maintaining protein homeostasis. Chapters describe methods that range from the in vitro analysis of enzyme cascades to real-time in vivo studies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Proteostasis: Methods and Protocols, Second Edition aims to be a useful practical guide to researches to help further their study in this field.

This volume is a collection of detailed protocols describing state-of-art approaches that will facilitate the understanding of protein homeostasis in plant stress responses and development. Plant Proteostasis: Methods and Protocols is broken into four parts focusing on the study of ubiquitin-dependent post-translational modifications, protocols focused on Ubl post-translational modifications, protein homeostasis, and protocols for the in silico analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Proteostasis: Methods and Protocols aims to address next challenges in agriculture such as precision horticulture.

Science and sensuality meet in The Greek Diet, your guide to losing weight while looking and eating like a deity, from the "Martha Stewart of Greece," world-famous chef Maria Loi, and health journalist and former Olympic athlete Sarah Toland. Grounded in the Mediterranean lifestyle and developed by world-renowned Greek chef Maria Loi, The Greek Diet combines classic Mediterranean ingredients like olive oil, yogurt, and honey in delicious, healthy recipes that satisfy the soul and the palete. New research shows that the Mediterranean diet is the healthiest food plan in the world, and is especially noted for its positive effects on heart health. With The Greek Diet, you eat to enjoy yourself, just like the ancient Greek gods. There is no starving, no long, grueling hours at the gym, and no restrictive plans eliminating carbs, dairy, caffeine, or alcohol. Structured around the 12 food pillars of the traditional Greek diet, The Greek Diet includes 100 healthy, authentic, sensual Greek recipes that use delicious unprocessed ingredients, as well as tips for incorporating easy exercises and improved sleep-both metabolism boosters-into your Greek lifestyle. Sprinkled throughout the book are charming and insightful anecdotes from the authors that add flavor and fun. There are also several different meal plans to personalize your journey and help you lose the weight you need while enjoying the foods you love, including a kick-start plan to shed pounds quickly and safely and jumpstart your journey to a slimmer, healthier, happier you.