|
Showing 1 - 9 of
9 matches in All Departments
An Up-to-Date Physical Science Toolbox for Probing Biology
Biophysics: Tools and Techniques for the Physics of Life covers the
experimental, theoretical and computational tools and techniques of
biophysics. It addresses the purpose, science, and application of
all physical science instrumentation, theoretical analysis and
biophysical computational methods used in current research labs.
The book first presents the historical background, concepts, and
motivation for using a physical science toolbox to understand
biology. It then familiarizes undergraduate students from the
physical sciences with essential biological knowledge. The text
subsequently focuses on experimental biophysical techniques that
primarily detect biological components or measure/control
biological forces. The author describes the science and application
of key tools used in imaging, detection, general quantitation, and
biomolecular interaction studies, which span multiple length and
time scales of biological processes both in the test tube and in
the living organism. Moving on to theoretical and computational
biophysics tools, the book presents analytical mathematical methods
and numerical simulation approaches for tackling challenging
biological questions including exam-style questions at the end of
each chapter as well as step-by-step solved exercises. It concludes
with a discussion of the future of this exciting field. Future
innovators will need to be trained in multidisciplinary science to
be successful in industry, academia, and government support
agencies. Addressing this challenge, this textbook educates future
leaders on the development and application of novel physical
science approaches to solve complex problems linked to biological
questions. Features: Provides the full, modern physical science
toolbox of experimental, theoretical and computational techniques,
such as bulk ensemble methods, single-molecule tools, live-cell and
test tube methods, pencil-on-paper theory approaches and
simulations Incorporates worked examples for the most popular
physical science tools, including full diagrams and a summary of
the science involved in the application of the tool Reinforces the
understanding of key concepts and biological questions A solutions
manual is available upon qualifying course adoption. Mark C. Leake
holds the Anniversary Chair of Biological Physics, and is
Coordinator of the Physics of Life Group at the University of York,
and Chair of the UK Physics of Life Network PoLNET. He heads an
interdisciplinary research team in the field of single-molecule
biophysics using cutting-edge biophotonics, state-of-the-art
genetics and advanced computational and theory tools. His work is
highly cited, and he has won many fellowships and prizes.
An Up-to-Date Physical Science Toolbox for Probing Biology
Biophysics: Tools and Techniques for the Physics of Life covers the
experimental, theoretical and computational tools and techniques of
biophysics. It addresses the purpose, science, and application of
all physical science instrumentation, theoretical analysis and
biophysical computational methods used in current research labs.
The book first presents the historical background, concepts, and
motivation for using a physical science toolbox to understand
biology. It then familiarizes undergraduate students from the
physical sciences with essential biological knowledge. The text
subsequently focuses on experimental biophysical techniques that
primarily detect biological components or measure/control
biological forces. The author describes the science and application
of key tools used in imaging, detection, general quantitation, and
biomolecular interaction studies, which span multiple length and
time scales of biological processes both in the test tube and in
the living organism. Moving on to theoretical and computational
biophysics tools, the book presents analytical mathematical methods
and numerical simulation approaches for tackling challenging
biological questions including exam-style questions at the end of
each chapter as well as step-by-step solved exercises. It concludes
with a discussion of the future of this exciting field. Future
innovators will need to be trained in multidisciplinary science to
be successful in industry, academia, and government support
agencies. Addressing this challenge, this textbook educates future
leaders on the development and application of novel physical
science approaches to solve complex problems linked to biological
questions. Features: Provides the full, modern physical science
toolbox of experimental, theoretical and computational techniques,
such as bulk ensemble methods, single-molecule tools, live-cell and
test tube methods, pencil-on-paper theory approaches and
simulations Incorporates worked examples for the most popular
physical science tools, including full diagrams and a summary of
the science involved in the application of the tool Reinforces the
understanding of key concepts and biological questions A solutions
manual is available upon qualifying course adoption. Mark C. Leake
holds the Anniversary Chair of Biological Physics, and is
Coordinator of the Physics of Life Group at the University of York,
and Chair of the UK Physics of Life Network PoLNET. He heads an
interdisciplinary research team in the field of single-molecule
biophysics using cutting-edge biophotonics, state-of-the-art
genetics and advanced computational and theory tools. His work is
highly cited, and he has won many fellowships and prizes.
This volume details a valuable collection of protocols and reviews,
such as emerging experimental and theoretical approaches. These
approaches have resulted in a substantial improvement in the
understanding of chromosome architecture. Chromosome Architecture:
Methods and Protocols guides readers through cutting-edge
interdisciplinary methods which allow for an understanding of
architecture of chromosomes with exceptionally enhanced resolution,
both in terms of space and time. Written in the highly successful
Methods in Molecular Biology series format, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and tips on troubleshooting and avoiding
known pitfalls. Authoritative and cutting-edge, Chromosome
Architecture: Methods and Protocols aims to ensure successful
results in the further study of this vital field.
This book describes modern biophysical techniques that enable us to
understand and examine dynamic processes of infection at the
molecular level. Cutting-edge research articles, laboratory
protocols, case studies and up-to-date reviews cover topics such as
single-molecule observation of DNA replication repair pathways in
E. coli; evolution of drug resistance in bacteria; restriction
enzymes as barriers to horizontal gene transfer in Staphylococcus
aureus; infectious and bacterial pathogen biofilms; killing
infectious pathogens through DNA damage; bacterial surfaces in
host-pathogen interactions; bacterial gene regulation by
riboswitches; transcription regulation in enterobacterial
pathogens; the bacterial flagellar motor; initial surface
colonization by bacteria; Salmonella Typhi host restrictions; as
well as monitoring proton motive force in bacteria; microbial
pathogens using digital holography; mathematical modelling of
microbial pathogen motility; neutron reflectivity in studying
bacterial membranes; force spectroscopy in studying infection and
4D multi-photon imaging to investigate immune responses. The focus
is on the development and application of complex techniques and
protocols at the interface of life sciences and physics, which
increase the physiological relevance of biophysical investigations.
This volume details a valuable collection of protocols and reviews,
such as emerging experimental and theoretical approaches. These
approaches have resulted in a substantial improvement in the
understanding of chromosome architecture. Chromosome Architecture:
Methods and Protocols guides readers through cutting-edge
interdisciplinary methods which allow for an understanding of
architecture of chromosomes with exceptionally enhanced resolution,
both in terms of space and time. Written in the highly successful
Methods in Molecular Biology series format, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and tips on troubleshooting and avoiding
known pitfalls. Authoritative and cutting-edge, Chromosome
Architecture: Methods and Protocols aims to ensure successful
results in the further study of this vital field.
This book describes modern biophysical techniques that enable us to
understand and examine dynamic processes of infection at the
molecular level. Cutting-edge research articles, laboratory
protocols, case studies and up-to-date reviews cover topics such as
single-molecule observation of DNA replication repair pathways in
E. coli; evolution of drug resistance in bacteria; restriction
enzymes as barriers to horizontal gene transfer in Staphylococcus
aureus; infectious and bacterial pathogen biofilms; killing
infectious pathogens through DNA damage; bacterial surfaces in
host-pathogen interactions; bacterial gene regulation by
riboswitches; transcription regulation in enterobacterial
pathogens; the bacterial flagellar motor; initial surface
colonization by bacteria; Salmonella Typhi host restrictions; as
well as monitoring proton motive force in bacteria; microbial
pathogens using digital holography; mathematical modelling of
microbial pathogen motility; neutron reflectivity in studying
bacterial membranes; force spectroscopy in studying infection and
4D multi-photon imaging to investigate immune responses. The focus
is on the development and application of complex techniques and
protocols at the interface of life sciences and physics, which
increase the physiological relevance of biophysical investigations.
This detailed new edition collects cutting-edge laboratory
protocols, techniques, and applications in use by some of the
leading international experts in the broad field of chromosome
architecture. The book emphasizes the increasing physiological
relevance of chromosome architecture investigation, manifest both
through application of more complex bottom-up assays in vitro as
well as through maintaining the native physiological context
through the investigation of living, functional cells. In addition,
the chapters reflect the dramatic improvement in the length scale
of precision by utilizing single-molecule approaches, both for
imaging the DNA content of chromosome and proteins that bind to DNA
as well as using methods that can controllably manipulate single
DNA molecules, and the use of advanced computational methods and
mathematical analysis is also featured. Written for the highly
successful Methods in Molecular Biology series, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and tips on troubleshooting and avoiding
known pitfalls. Authoritative and up-to-date, Chromosome
Architecture: Methods and Protocols, Second Edition is an ideal
guide for researchers working in this dynamic area of study.
This detailed new edition collects cutting-edge laboratory
protocols, techniques, and applications in use by some of the
leading international experts in the broad field of chromosome
architecture. The book emphasizes the increasing physiological
relevance of chromosome architecture investigation, manifest both
through application of more complex bottom-up assays in vitro as
well as through maintaining the native physiological context
through the investigation of living, functional cells. In addition,
the chapters reflect the dramatic improvement in the length scale
of precision by utilizing single-molecule approaches, both for
imaging the DNA content of chromosome and proteins that bind to DNA
as well as using methods that can controllably manipulate single
DNA molecules, and the use of advanced computational methods and
mathematical analysis is also featured. Written for the highly
successful Methods in Molecular Biology series, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and tips on troubleshooting and avoiding
known pitfalls. Authoritative and up-to-date, Chromosome
Architecture: Methods and Protocols, Second Edition is an ideal
guide for researchers working in this dynamic area of study.
Recent advances in single molecule science have presented a new
branch of science: single molecule cellular biophysics, combining
classical cell biology with cutting-edge single molecule
biophysics. This textbook explains the essential elements of this
new discipline, from the state-of-the-art single molecule
techniques to real-world applications in unravelling the inner
workings of the cell. Every effort has been made to ensure the text
can be easily understood by students from both the physical and
life sciences. Mathematical derivations are kept to a minimum
whilst unnecessary biological terminology is avoided and text boxes
provide readers from either background with additional information.
100 end-of-chapter exercises are divided into those aimed at
physical sciences students, those aimed at life science students
and those that can be tackled by students from both disciplines.
The use of case studies and real research examples make this
textbook indispensable for undergraduate students entering this
exciting field.
|
|