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In the early 1990s, almost 200 yr after Edward Jenner demonstrated
the effectiveness of the smallpox vaccine, a new paradigm for
vaccination emerged. The conventional method of vaccination
required delivery of whole pathogens or structural subunits, but in
this new approach, DNA or genetic information was administered to
elicit an immunological response. Once it was observed that plasmid
DNA delivered in vivo led to production of an encoded transgene
(1), two ground-breaking studies demonstrated that immunological
responses could be generated against antigenic transgenes via
plasmid DNA delivered by DNA vaccination (as this approach is
called) (2,3). The appe- ance of this new vaccination strategy
coincided with advances in molecular biology, which provided new
tools to study and manipulate the basic elements of an organism's
genome and also could also be applied to the design and production
of DNA vaccines. DNA Vaccines is a major updated and enhancement of
the first edition. It reviews state-of-the-art methods in DNA
vaccine technology, with chapters describing DNA vaccine design,
delivery systems, adjuvants, current appli- tions, methods of
production, and quality control. Consistent with the approach of
the Methods in Molecular Medicine series, these chapters contain
detailed practical procedures on the latest DNA vaccine technology.
The enthusiasm for DNA vaccine technology is made clear by the
number of research studies published on this topic since the
mid-1990s.
In this completely revised and updated second edition of DNA
Vaccines: Methods and Protocols, W. Mark Saltzman presents a
comprehensive collection of DNA vaccine protocols, written by
leading experts in the groundbreaking field of DNA vaccination.
Divided into five sections, this volume contains state-of-the-art
and practical procedures on the latest DNA vaccine technology. Part
I contains DNA vaccine design protocols, focusing on methods that
achieve optimal expression in host cells. Part II is dedicated to
presenting methods for DNA delivery, and covers both the range of
administration methods available for vaccine administration and a
variety of techniques for improving the efficiency of delivery into
cells. Part III discusses current available methods, including
adjuvant and prime-boost approaches, for enhancing the potency of
DNA vaccines. Part IV describes several key areas of application in
the field, including allergy, avoidance of autoimmunity, and
neonate and infant vaccine response. DNA Vaccines: Methods and
Protocols, Second Edition concludes with a review of protocols for
vaccine production and purification, and applicable quality control
methods.
This collection of up-to-date protocols demonstrates the
tremendous progress that has been made in the field of DNA
vaccination since the initial appearance of this new vaccination
strategy. Given current enthusiasm for the magnificent potential
for this technology among the scientific community, researchers
will find tremendous value in this updated and enhanced molecular
methods guide.
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