Since the publication of the best-selling first edition, much has been discovered about Saccharomyces cerevisiae, the single-celled fungus commonly known as baker's yeast or brewer's yeast that is the basis for much of our understanding of the molecular and cellular biology of eukaryotes. This wealth of new research data demands our attention and requires a reference that updates our current knowledge. Completely rewritten, updated, and expanded, Metabolism and Molecular Physiology of Saccharomyces cerevisiae, 2nd Edition provides a modern account of the metabolism and physiology of this important organism. All chapters have been thoroughly revised, and two new chapters on ageing and on the molecular organization and biogenesis of the cell wall have been added to highlight new areas of research. The book also includes coverage of morphogenesis, protein trafficking, lipids and membranes, and stress responses. Written by a team of internationally recognized experts, this authoritative second edition is a leading reference for bioscientists across many disciplines as well as novices interested in the latest developments in this complex field.

Fungi are a diverse group of organisms, studied widely because of their commercial importance in biotechnology, agriculture and medicine, and because they provide simple model systems for illuminating the eukaryotic mode of life. The development of the techniques of molecular biology has opened up new areas of mycological research, with profound consequences such as the sequencing of the first entire eukaryotic genome. This book focuses on the application of molecular and cell biology to mycology and presents examples of how these approaches are elucidating long-held problems in areas as diverse as animal and plant pathology, protein expression and evolution. Aimed at advanced undergraduates taking courses in mycology, applied biology, biotechnology, fungal biology and fungal genetics, this textbook will also serve postgraduates needing an introduction to modern fungal research.

Fungi are a diverse group of organisms, studied widely because of their commercial importance in biotechnology, agriculture, and medicine, and because they provide simple model systems for illuminating the eukaryotic mode of life. The advancement of techniques in molecular biology has opened up new areas of mycological research, with important consequences such as the sequencing of the first entire eukaryotic genome. This book focuses on the application of molecular and cell biology to mycology and presents examples of how these approaches are elucidating long-held problems in areas as diverse as animal and plant pathology, protein expression, and evolution. Aimed at advanced undergraduates taking courses in mycology, applied biology, biotechnology, fungal biology and fungal genetics, this textbook will also serve postgraduates needing an introduction to modern fungal research.

Provides a grounding in the experimental techniques applicable to the discipline of biotechnology. The introductory section in the text describes procedures for analysis of inorganic and organic materials, strain maintenance and fundamental experiments in gene manipulation. Other chapters deal with fermentation techniques, purification methods for substances of interest, preparation of microbial sensors and the demonstration of oil degradation by bacteria. The final chapter deals with statistical planning of experiments and scale-up methods.