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This second edition volume expands on the previous edition with
descriptions of recent developments in the field. The chapters in
this book cover topics such as monoclonal antibodies for the
treatment of melanoma; production and purification of human
monoclonal antibodies; humanization and optimization of monoclonal
antibodies; rapid chimerization of monoclonal antibodies; epitope
mapping via phage display from single gene libraries; recombinant
antibodies made by combining phage and yeast display selections;
production of stabilized antibody fragments in the E. coli
bacterial cytoplasm and transfected mammalian cells; and analysis
of CAR T cells. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls. Unique and
thorough, Human Monoclonal Antibodies: Methods and Protocols,
Second Edition is a valuable tool for novice and expert researchers
interested in learning more about this evolving field.
The introduction of monoclonal antibodies revolutionized
immunology. The development of human monoclonal antibodies was
inspired primarily by the enormous clinical benefits promised by
these reagents which can be used as anti-inflammatory reagents,
anti-tumor reagents and reagents for passive immunization in a
variety of pathologies. Human Monoclonal Antibodies: Methods and
Protocols presents technical protocols of cellular and molecular
methods for the production, purification and application of human
monoclonal antibodies, as well as review articles on related topics
of human monoclonal and polyclonal antibodies. Written in the
successful Methods in Molecular Biology series format, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible protocols, and notes on troubleshooting and avoiding
known pitfalls. Authoritative and easily accessible, Human
Monoclonal Antibodies: Methods and Protocols seeks to serve both
professionals and novices with its well-honed methodologies which
will prove invaluable in a clinical setting.
The introduction of monoclonal antibodies revolutionized
immunology. The development of human monoclonal antibodies was
inspired primarily by the enormous clinical benefits promised by
these reagents which can be used as anti-inflammatory reagents,
anti-tumor reagents and reagents for passive immunization in a
variety of pathologies. Human Monoclonal Antibodies: Methods and
Protocols presents technical protocols of cellular and molecular
methods for the production, purification and application of human
monoclonal antibodies, as well as review articles on related topics
of human monoclonal and polyclonal antibodies. Written in the
successful Methods in Molecular Biology series format, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible protocols, and notes on troubleshooting and avoiding
known pitfalls. Authoritative and easily accessible, Human
Monoclonal Antibodies: Methods and Protocols seeks to serve both
professionals and novices with its well-honed methodologies which
will prove invaluable in a clinical setting.
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