The introduction of monoclonal antibodies revolutionized
immunology. The development of human monoclonal antibodies was
inspired primarily by the enormous clinical benefits promised by
these reagents which can be used as anti-inflammatory reagents,
anti-tumor reagents and reagents for passive immunization in a
variety of pathologies. Human Monoclonal Antibodies: Methods and
Protocols presents technical protocols of cellular and molecular
methods for the production, purification and application of human
monoclonal antibodies, as well as review articles on related topics
of human monoclonal and polyclonal antibodies. Written in the
successful Methods in Molecular Biology series format, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible protocols, and notes on troubleshooting and avoiding
known pitfalls. Authoritative and easily accessible, Human
Monoclonal Antibodies: Methods and Protocols seeks to serve both
professionals and novices with its well-honed methodologies which
will prove invaluable in a clinical setting.
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