Biological control has become an attractive alternative strategy for the control of plant diseases to reduce the excessive use of agrochemicals and its health hazards. But a significant gap still exists between basic research involving the discovery of a biocontrol agent and its development and implementation under commercial conditions. Because BCAs (unlike chemical a.m.) need to establish, colonize, survive and perform their metabolic activity to control diseases. In order to move a biocontrol agent from the laboratory to the market place requires many different disciplines and people with a variety of expertise. Research can stimulate the development of commercial biocontrol agents. Chapter 16 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Climate Change and Mycotoxins highlights the importance of the continuous study of climate change impacts on mycotoxigenic fungi and their toxins in food and feed crops. Changing climate conditions across every geographical zone greatly affect rainfall, temperature and concentration of greenhouse gases leading to loss in yield and quality of food crops. In outstanding contributions, the authors compile current evidence on the influence of climate change on mycotoxigenic fungi and mycotoxins in food crops pre- and postharvest and during storage of food and animal feed. The chemistry and biology of toxin production is revised and an outlook on control and prevention of the toxin's impact on food and animal feed is given. The editors recommend this book to mycologists, mycotoxicologists, pathologists, epidemiologists, toxicologists, physicians, veterinarians, nutritionists, the food and feed industries, legislators, analytical chemists, microbiologists, or students of these fields. * Unique compilation on the impact of climate change on mycotoxins based on observed trends over the last 10 years. * Special focus on the implications for food and feed safety. * Latest advances on prediction and prevention of mycotoxin threats to human and animal health. About the Editors Luis M. Botana Is a full Professor of Pharmacology at the University of Santiago, from 2004-2012 director of the Department of Pharmacology and former Fogarty Fellow at the School of Medicine of the Johns Hopkins University. He has been director of the European Reference Laboratory for Marine Toxins from 2004 to 2009. He is author of 25 international patents, over 300 scientific papers and editor of 10 international books. Maria J. Sainz Is an associate Professor of Agriculture and Forage Production and Conservation at the University of Santiago de Compostela. She has been a visiting scientist at the Rothamsted Experimental Station and for ten years head of the department of Plant Production. Her research interests focus on fungal pathogen detection and diagnostics, mycorrhizal fungi in crop protection and production, and mycotoxigenic fungi and mycotoxins on forage crops and animal feed.

There is increasing interest in the use of fungi for the control of pests, weeds and diseases. This book brings together perspectives from pathology, ecology, genetics, physiology, production technology, to address the use of fungi as biological control agents.

The main objective of this study was to determine and understand how environmental factors influence growth and the regulation of AFB1 production, and to gain knowledge of the correlation of ecophysiological conditions with toxin gene expression of the aflatoxin genes in relation to phenotypic production. In this study the following studies have been carried out: Effect of combinations of water activity x temperature interactions on growth, and sporulation, and partitioning of AFB1 into spores, mycelium and medium using A. flavus NRRL 3357. Use of a microarray and real-timePCR to examine the effects of aw x temperature interactions on a conducive YES and to examine the relationship between the expression of the genes and phenotypic aflatoxin production.A relative quantification system (RQ-PCR) was used to monitor and correlate the temporal activity of the aflD gene of Aspergillus flavus using real-time PCR in relation to phenotypic AFB1 production and populations of A. flavus in stored peanuts. To examine the potential of three designed siRNA sequences to target the mRNA sequence of the aflD gene for using RNA silencing technology to control aflatoxin production.

This collection features five peer-reviewed literature reviews on mycotoxin control in agriculture. The first chapter reviews advances in post-harvest detection and control of fungal contaminants in cereals. It examines abiotic factors affecting spoilage, methods for early detection of contamination and the range control measures for preventing toxin growth. The second chapter focuses on post-harvest storage and handling practices of barley grain and how these methods can be used to mitigate mycotoxin issues. The chapter also reviews the various mycotoxins and fungi that are associated with barley. The third chapter considers the current strategies available to prevent mycotoxin contamination in groundnut cultivation, focussing on peanuts. It also covers models that predict contamination, as well as the challenges associated with research and quantification of aflatoxin. The fourth chapter presents an overview of the current understanding of mycotoxin contamination of cocoa. The chapter summarises the various methods available to aid detection of mycotoxins and control further contamination. The final chapter addresses the critical safety issue of mycotoxin contamination of food waste planned for re-use. It reviews factors affecting mycotoxin growth and the particular problem of masked mycotoxins.

Despite an upsurge in national and international debate on environmental issues since the Earth Summit in Rio de Janeiro in 1992, fungi, vital to the functioning of terrestrial and aquatic ecosystems, are rarely mentioned. This volume helps redress this imbalance by considering fungi in the context of the impact of humankind's activity on their habitats. The presentation of experimental evidence is a major feature of the volume. Contributions on the effects of global warming, UV-B radiation, atmospheric and terrestrial pollutants, deforestation in the tropics, loss of biodiversity, genetic engineering and chaos theory ensure a topical and balanced coverage, with both ecological and physiological viewpoints being represented. This timely review will be of interest to all mycologists and those ecologists concerned with environmental change.