The main objective of this study was to determine and understand
how environmental factors influence growth and the regulation of
AFB1 production, and to gain knowledge of the correlation of
ecophysiological conditions with toxin gene expression of the
aflatoxin genes in relation to phenotypic production. In this study
the following studies have been carried out: Effect of combinations
of water activity x temperature interactions on growth, and
sporulation, and partitioning of AFB1 into spores, mycelium and
medium using A. flavus NRRL 3357. Use of a microarray and
real-timePCR to examine the effects of aw x temperature
interactions on a conducive YES and to examine the relationship
between the expression of the genes and phenotypic aflatoxin
production.A relative quantification system (RQ-PCR) was used to
monitor and correlate the temporal activity of the aflD gene of
Aspergillus flavus using real-time PCR in relation to phenotypic
AFB1 production and populations of A. flavus in stored peanuts. To
examine the potential of three designed siRNA sequences to target
the mRNA sequence of the aflD gene for using RNA silencing
technology to control aflatoxin production.
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