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This timely book opens up new avenues in the development of highly
sensitive and specific fluorometric and sensor methods based on the
NIR laser techniques, allowing detection down to the single
molecule level. Most important are NIR laser diodes in combination
with NIR dyes, which facilitates the automation and miniaturisation
of reliable UV/vis and fluorescence spectroscopic and immunoassay
in situ measurements. This permits, for example, analyses of pH
values, metal ions, pollutants, membranes, proteins, living cells
and the DNA genetic code. Furthermore, the book describes new
applications of NIR dyes in high technology areas, such as
photochemistry, molecular biology, clinical chemistry, tumour
therapy, laser physics, nonlinear optics, laser sensitive optical
recording techniques, optical disks, compact disks, laser printers,
optical cards, photoengraving, transparent bar coding, forgery
prevention, photoresists, spectrally sensitised photographic
materials, thermal transfer printing, and heat shielding materials.
A compilation of challenging information for scientists and
engineers interested in high technology developments and
applications.
"Provides analytical chemists and biomedical scientists with an
excellent summary of progress...This is a book that can be
recommended to all analytical scientists interested in
fluorimetry." (Analytical Chimica Acta) "This is a useful overview
and gives the nonspecialist a feeling for the advantages and
limitations of the methods. Overall this book is a worthwhile read
and a good source of references." (TRAC)
The book is divided into chapters on new methods, new appli-
cations, fluorescence immunoassays, fluorometric analysis and
fluorescence spectroscopy in biomedical sciences. Specific topics
are fluorescence spectroscopy using synchrotron radiation,
picosecond fluorescence spectroscopy, fluorescence microscopy,
fluorescence scattering by synthetic polymers, fluorescence
immunoassays, fluorescence for environmental monitoring,
fluorescence in flow injection analysis, hydro-geological studies,
fluorescence of proteins, lipids and membranes, cell fluorescence,
calcium transients.
Optical sensor technology has reached a level of technological
maturity that makes it a promising candidate for applications to
specific sensing challenges including those in environmental
monitoring, in process control (particularly in biotechnology), in
clinical assays where low-cost one-way sensing elements are needed,
and in other areas. Optical sensors can be used as fiber optic
microsensors, as planar coatings in bioreactors, in microtiterplate
format, in disposable single-shot devices, and as planar membranes
that can be imaged using sensitive cameras. The spectral range
extends from the UV to the infrared, and from absorption to
emission and to surface plasmon resonance. Hence, a variety of
schemes are conceivable, and this first volume of the Springer
Series on Chemical Sensors and Biosensors gives a state-of-the-art
description of this highly sophisticated but very promising
technology.
This timely book opens up new avenues in the development of highly
sensitive and specific fluorometric and sensor methods based on the
NIR laser techniques, allowing detection down to the single
molecule level. Most important are NIR laser diodes in combination
with NIR dyes, which facilitates the automation and miniaturisation
of reliable UV/vis and fluorescence spectroscopic and immunoassay
in situ measurements. This permits, for example, analyses of pH
values, metal ions, pollutants, membranes, proteins, living cells
and the DNA genetic code. Furthermore, the book describes new
applications of NIR dyes in high technology areas, such as
photochemistry, molecular biology, clinical chemistry, tumour
therapy, laser physics, nonlinear optics, laser sensitive optical
recording techniques, optical disks, compact disks, laser printers,
optical cards, photoengraving, transparent bar coding, forgery
prevention, photoresists, spectrally sensitised photographic
materials, thermal transfer printing, and heat shielding materials.
A compilation of challenging information for scientists and
engineers interested in high technology developments and
applications.
This first volume in the new Fluorescence Spectroscopy series brings together fundametnal and applied research from this highly interdisciplinary field ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, senso applications, confocal microscopy and protein-protein interactions. This casefully edited collection of state-of-the-art articles will serve as an invaluable tool for pactitioners and ..... and give them inspiration for new developments and applications.
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