This work provides information on the detection, identification, and differentiation of all microbial plant pathogens - presenting modern protocols for rapid diagnosis of diseases based on biological, physical, chemical and molecular properties. It contains methods for the selection of disease-free seeds and vegetatively propagated planting materials and quarantine techniques for screening newly introduced plant materials.

Soil has a versatile role in supporting the development of a wide range of organisms, including plants and microorganisms. Soilborne pathogens and root diseases are the primary limiting factor in many crops and tend to be very difficult to control. This first volume of a two-volume set introduces disease-causing microorganisms including oomycetes, fungi, bacteria, and viruses found in soils. It focuses on the biology, detection, and identification of soilborne bacterial, fungal, and viral plant pathogens. Volume two provides information on ecology and epidemiology of soilborne microbial plant pathogens and strategies applicable to manage diseases. Chapters cover exclusion and prevention strategies; improvement of host plant resistance; biological management; application of chemicals; and integration of disease management strategies.

Crop disease management strategies revolve around the principles of exclusion, eradication and immunization. Cultural practices are aimed at preventing or reducing the accumulation of pathogen population (inoculum). Development of cultivars with genetic resistance by transgressing resistance gene(s) through traditional breeding procedures or biotechnological techniques is the most effective and acceptable strategy, as it is environment-friendly and does not need any additional cost to the grower. Assessment of different grades of resistance of cultivars or genotypes to soilborne microbial pathogens has been possible by quantifying pathogen populations or their DNA contents in the test plants by applying biological and molecular methods. This second volume of a two-volume set focuses on the soilborne microbial plant pathogens and the diseases caused by them. The book provides information on ecology and epidemiology of soilborne microbial plant pathogens and various strategies applicable for effective management of diseases. Chapters cover exclusion and prevention strategies; improvement of host plant resistance; biological management; application of chemicals; and integration of these disease management strategies. Features Discusses various aspects of soilborne microbial plant pathogens to develop effective methods of managing diseases. Presents information on epidemiology and ecology of soilborne microbial plant pathogens. Facilitates the application of management strategies alone or in combination with others for effective suppression of disease development. Features information on application of biotic and abiotic biological control agents (BCAs) to suppress pathogen development either by directly acting on the pathogen(s) or indirectly by enhancing host resistance to the pathogens. Employs biotic and abiotic biocontrol agents either to replace or reduce the use of chemicals is an achievable approach for managing the soilborne microbial pathogens.

Soilborne microbial plant pathogens including oomycetes, fungi, bacteria and viruses cause several economically important destructive diseases and the symptoms of infection can be recognized only after the pathogen has invaded many tissues primarily vascular tissues of susceptible plants. This condition places formidable challenges in investigating different aspects of host-microbial pathogen interactions. Early detection of infection and precise identification, differentiation, and quantification of the microbial plant pathogens in plants, soil and water sources are essential requirements for development of effective tactics to reduce the incidence and spread of the diseases caused by them. As the microbial plant pathogens differ in their virulence and sensitivity to the environment and chemicals applied, it is imperative to assess the extent of variability in the concerned pathogens. This first volume of a two-volume set introduces disease-causing microorganisms including oomycetes, fungi, bacteria, and viruses found in soils. It focuses on the biology, detection, and identification of soilborne bacterial, fungal, and viral plant pathogens. This volume discusses various techniques based on biological, immunological and genetic properties of the pathogens indicating their advantages and limitations for selecting the appropriate technique to fulfill the requirements. Features: Presents techniques useful for detection, identification, quantification of microbial plant pathogens in plants, soil, and irrigation water from waterbodies. Highlights subversive activities of viruses, resulting in the breakdown of host defense systems. Discusses RNA silencing in infected plants by viruses and posttranscriptional gene silencing (PTGS) functioning as an endogenous mechanism in plants against virus infection. Presents information on methods of assessment of genetic variability and sensitivity of microbial plant pathogens to chemicals and adverse environmental conditions.

This work provides information on the detection, identification, and differentiation of all microbial plant pathogens - presenting modern protocols for rapid diagnosis of diseases based on biological, physical, chemical and molecular properties. It contains methods for the selection of disease-free seeds and vegetatively propagated planting materials and quarantine techniques for screening newly introduced plant materials.

Microbial plant pathogens causing qualitative and quantitative losses in all corps are present not only in the infected plants, but also in the environmental comprising of soil, water and air. The vectors present in the environment spread the microbial pathogens to short and/or long distances. Detection of microbial pathogens rapidly and reliably by employing suitable sensitive applicable for different ecosystems. The pathogens have to be identified precisely and differentiated and quantified to plan appropriate short- and long-term strategies to contain the incidence and spread of diseases induced by them. This book aims to present all relevant and latest information on the detection techniques based on the biological, biochemical, immunological and nucleic acid characteristics of microbial pathogens presents in the host plants, as well as in the natural substrates that support the survival and perpetuation of the pathogens.

The need for the development of techniques based on the characteristics of the viral proteins and genomic nucleic acids was realized in order to detect, identify, differentiate and quantify viruses in the infected plants/planting materials with or without symptoms of infection. Immunoassays have been successfully applied for the detection of viruses in crop and weed host plant species as well as in the vectors. Nucleic acid-based techniques have been demonstrated to be the most reliable and sensitive tests for detection, identification and differentiation of viruses and viroids present in plants and planting materials.. Inclusion of numerous protocols in appropriate chapters as appendix is a unique feature of this volume.

Morphological, biological, biochemical and physiological characteristics have been used for the detection, identification and differentiation of fungal pathogens up to species level. Tests based on biological characteristics are less consistent. Immunoassays have been shown to be effective in detecting fungal pathogens present in plants and environmental samples. Development of monoclonal antibody technology has greatly enhanced the sensitivity and specificity of detection, identification and differentiation of fungal species and varieties/strains. Nucleic acid-based techniques involving hybridization with or amplification of unique DNA have provided results rapidly and reliably. Presentation of a large number of protocols is a unique feature of this volume.

Studies on the phenomenon of plant pathogenesis (disease development) have been useful to have a deep insight into the interactions between host plant and the pathogen. Depending on the levels of susceptibility (compatibility) or resistance (incompatibility) of the host plant and virulence of the pathogen, disease development may progress, either leading to symptom expression or result in the suppression of pathogen proliferation. Molecular techniques have been applied to elucidate the nature of interactions between the gene products of the plant and pathogen at cellular and molecular levels. Successful evasion of host's surveillance system and subsequent activities of metabolites of the pathogen (enzymes and toxins) encoded by pathogen genes counteracting the effects of various defense-related antimicrobial compounds present already or produced by the host plants, after initiation of infection have been critically studied by applying various molecular techniques. In addition to studying various phases of disease development in individual plants, molecular methods have been demonstrated to be effective, in gathering data on various aspects of epidemiology under natural conditions where the interaction of pathogen with populations of plants is influenced significantly by the environmental conditions existing in different ecosystems. This volume focuses on the possibility of applying the knowledge on pathogenesis and molecular epidemiology to determine the vulnerable stages in the life cycles of the pathogens that can be disrupted to achieve more effective disease control.

The need for the development of techniques based on the characteristics of the viral proteins and genomic nucleic acids was realized in order to detect, identify, differentiate and quantify viruses in the infected plants/planting materials with or without symptoms of infection. Immunoassays have been successfully applied for the detection of viruses in crop and weed host plant species as well as in the vectors. Nucleic acid-based techniques have been demonstrated to be the most reliable and sensitive tests for detection, identification and differentiation of viruses and viroids present in plants and planting materials.. Inclusion of numerous protocols in appropriate chapters as appendix is a unique feature of this volume.

Morphological, biological, biochemical and physiological characteristics have been used for the detection, identification and differentiation of fungal pathogens up to species level. Tests based on biological characteristics are less consistent. Immunoassays have been shown to be effective in detecting fungal pathogens present in plants and environmental samples. Development of monoclonal antibody technology has greatly enhanced the sensitivity and specificity of detection, identification and differentiation of fungal species and varieties/strains. Nucleic acid-based techniques involving hybridization with or amplification of unique DNA have provided results rapidly and reliably. Presentation of a large number of protocols is a unique feature of this volume.

Microbial plant pathogens causing qualitative and quantitative losses in all corps are present not only in the infected plants, but also in the environmental comprising of soil, water and air. The vectors present in the environment spread the microbial pathogens to short and/or long distances. Detection of microbial pathogens rapidly and reliably by employing suitable sensitive applicable for different ecosystems. The pathogens have to be identified precisely and differentiated and quantified to plan appropriate short- and long-term strategies to contain the incidence and spread of diseases induced by them. This book aims to present all relevant and latest information on the detection techniques based on the biological, biochemical, immunological and nucleic acid characteristics of microbial pathogens presents in the host plants, as well as in the natural substrates that support the survival and perpetuation of the pathogens.

Investigations on various aspects of plant-pathogen interactions have the ultimate aim of providing information that may be useful for the development of effective crop disease management systems. Molecular techniques have accelerated the formulation of short- and long-term strategies of disease management. Exclusion and eradication of plant pathogens by rapid and precise detection and identification of microbial pathogens in symptomatic and asymptomatic plants and planting materials by employing molecular methods has been practiced extensively by quarantines and certification programs with a decisive advantage. Identification of sources of resistance genes, cloning and characterization of desired resistance genes and incorporation of resistance gene(s) into cultivars and transformation of plants with selected gene(s) have been successfully performed by applying appropriate molecular techniques. Induction of resistance in susceptible cultivars by using biotic and abiotic inducers of resistance is a practical proposition for several crops whose resistance levels could not be improved by breeding or transformation procedures. The risks of emergence of pathogen strains less sensitive or resistant to chemicals have been reduced appreciably by rapid identification of resistant strains and monitoring the occurrence of such strains in different geographical locations.

Studies on molecular biology of pathogens, infection process and disease resistance, have provided information essentially required to understand the vulnerable stages at which the pathogens can be tackled effectively and to adopt novel strategies to incorporate disease resistance genes from diverse sources and /or to induce resistance of cultivars with desirable agronomic attributes using biotic or abiotic agents. The nature of interaction between the gene products of the pathogen and plant appears to determine the outcome of the interaction resulting in either disease progression or suppression. Transgenic plants with engineered genes show promise for effective exploitation of this approach for practical application. Research efforts during the recent years to sequence the whole genomes of the pathogens and plants may lead to development of better ways of manipulating disease resistance mechanisms enabling the grower to achieve higher production levels and the consumer to enjoy safer food and agricultural products. Experimental protocols included in appropriate chapters will be useful for researchers and graduate students.

Investigations on various aspects of plant-pathogen interactions have the ultimate aim of providing information that may be useful for the development of effective crop disease management systems. Molecular techniques have accelerated the formulation of short- and long-term strategies of disease management. Exclusion and eradication of plant pathogens by rapid and precise detection and identification of microbial pathogens in symptomatic and asymptomatic plants and planting materials by employing molecular methods has been practiced extensively by quarantines and certification programs with a decisive advantage. Identification of sources of resistance genes, cloning and characterization of desired resistance genes and incorporation of resistance gene(s) into cultivars and transformation of plants with selected gene(s) have been successfully performed by applying appropriate molecular techniques. Induction of resistance in susceptible cultivars by using biotic and abiotic inducers of resistance is a practical proposition for several crops whose resistance levels could not be improved by breeding or transformation procedures. The risks of emergence of pathogen strains less sensitive or resistant to chemicals have been reduced appreciably by rapid identification of resistant strains and monitoring the occurrence of such strains in different geographical locations.

Studies on the phenomenon of plant pathogenesis (disease development) have been useful to have a deep insight into the interactions between host plant and the pathogen. Depending on the levels of susceptibility (compatibility) or resistance (incompatibility) of the host plant and virulence of the pathogen, disease development may progress, either leading to symptom expression or result in the suppression of pathogen proliferation. Molecular techniques have been applied to elucidate the nature of interactions between the gene products of the plant and pathogen at cellular and molecular levels. Successful evasion of hosta (TM)s surveillance system and subsequent activities of metabolites of the pathogen (enzymes and toxins) encoded by pathogen genes counteracting the effects of various defense-related antimicrobial compounds present already or produced by the host plants, after initiation of infection have been critically studied by applying various molecular techniques. In addition to studying various phases of disease development in individual plants, molecular methods have been demonstrated to be effective, in gathering data on various aspects of epidemiology under natural conditions where the interaction of pathogen with populations of plants is influenced significantly by the environmental conditions existing in different ecosystems. This volume focuses on the possibility of applying the knowledge on pathogenesis and molecular epidemiology to determine the vulnerable stages in the life cycles of the pathogens that can be disrupted to achieve more effective disease control.

Studies on molecular biology of pathogens, infection process and disease resistance, have provided information essentially required to understand the vulnerable stages at which the pathogens can be tackled effectively and to adopt novel strategies to incorporate disease resistance genes from diverse sources and /or to induce resistance of cultivars with desirable agronomic attributes using biotic or abiotic agents. The nature of interaction between the gene products of the pathogen and plant appears to determine the outcome of the interaction resulting in either disease progression or suppression. Transgenic plants with engineered genes show promise for effective exploitation of this approach for practical application. Research efforts during the recent years to sequence the whole genomes of the pathogens and plants may lead to development of better ways of manipulating disease resistance mechanisms enabling the grower to achieve higher production levels and the consumer to enjoy safer food and agricultural products. Experimental protocols included in appropriate chapters will be useful for researchers and graduate students.

Healthy seeds and propagules are the basic requirement for producing good grains, fruits and vegetables needed for human survival and perpetuation. Dispersal of microbial plant pathogens via seeds and propagules has assumed more importance than other modes of dispersal, as infected seeds and propagules have the potential to become the primary sources of carrying pathogen inoculum for subsequent crops. Several diseases transmitted through seeds and propagules have been shown to have the potential to damage economies as a result of huge quantitative and qualitative losses in numerous crops. Hence, it is essential to rapidly detect, identify and differentiate the microbial plant pathogens present in seeds and propagules precisely and reliably, using sensitive techniques. Microbial Plant Pathogens: Detection and Management in Seeds and Propagules provides a comprehensive resource on seed-borne and propagule-borne pathogens. Information on the biology of microbial pathogens, including genetic diversity, infection process and survival mechanisms of pathogens and epidemiology of diseases caused by them, are discussed critically and in detail to highlight weak links in the life cycles of the pathogens. Development of effective disease management systems, based on the principles of exclusion and eradication of pathogens and immunization of crop plants to enhance the levels of resistance of cultivars to diseases, has been effective to keep the pathogens at bay. The need for production of disease-free seeds/propagules has been emphasized to prevent the carryover of the inoculum to the next crop or introduction of the pathogens to other locations. Effectiveness of adopting simple cultural practices and development of cultivars resistant to diseases through traditional breeding methods or biotechnological approach have resulted in reducing the pathogen inoculum and disease incidence. Although application of different chemicals may reduce the disease incidence effectively, biological management of crop diseases, employing potential biological control agents have to be preferred to preserve the agroecosystems. Greater efforts have to be made to integrate compatible strategies to enhance the effectiveness of diseases management systems. Protocols appended at the end of relevant chapters form a unique feature of this book to enable the researchers to fine-tune their projects. This 2 volume set provides comprehensive and updated information about the economically-important groups of microbial plant pathogens carried by seed and propagules. Graduate students, researchers and teachers of plant pathology, plant protection, microbiology, plant breeding and genetics, agriculture and horticulture, as well as certification and quarantine personnel will find the information presented in this book useful.

Crop disease management strategies revolve around the principles of exclusion, eradication and immunization. Cultural practices are aimed at preventing or reducing the accumulation of pathogen population (inoculum). Development of cultivars with genetic resistance by transgressing resistance gene(s) through traditional breeding procedures or biotechnological techniques is the most effective and acceptable strategy, as it is environment-friendly and does not need any additional cost to the grower. Assessment of different grades of resistance of cultivars or genotypes to soilborne microbial pathogens has been possible by quantifying pathogen populations or their DNA contents in the test plants by applying biological and molecular methods. This second volume of a two-volume set focuses on the soilborne microbial plant pathogens and the diseases caused by them. The book provides information on ecology and epidemiology of soilborne microbial plant pathogens and various strategies applicable for effective management of diseases. Chapters cover exclusion and prevention strategies; improvement of host plant resistance; biological management; application of chemicals; and integration of these disease management strategies. Features Discusses various aspects of soilborne microbial plant pathogens to develop effective methods of managing diseases. Presents information on epidemiology and ecology of soilborne microbial plant pathogens. Facilitates the application of management strategies alone or in combination with others for effective suppression of disease development. Features information on application of biotic and abiotic biological control agents (BCAs) to suppress pathogen development either by directly acting on the pathogen(s) or indirectly by enhancing host resistance to the pathogens. Employs biotic and abiotic biocontrol agents either to replace or reduce the use of chemicals is an achievable approach for managing the soilborne microbial pathogens.

Soilborne microbial plant pathogens including oomycetes, fungi, bacteria and viruses cause several economically important destructive diseases and the symptoms of infection can be recognized only after the pathogen has invaded many tissues primarily vascular tissues of susceptible plants. This condition places formidable challenges in investigating different aspects of host-microbial pathogen interactions. Early detection of infection and precise identification, differentiation, and quantification of the microbial plant pathogens in plants, soil and water sources are essential requirements for development of effective tactics to reduce the incidence and spread of the diseases caused by them. As the microbial plant pathogens differ in their virulence and sensitivity to the environment and chemicals applied, it is imperative to assess the extent of variability in the concerned pathogens. This first volume of a two-volume set introduces disease-causing microorganisms including oomycetes, fungi, bacteria, and viruses found in soils. It focuses on the biology, detection, and identification of soilborne bacterial, fungal, and viral plant pathogens. This volume discusses various techniques based on biological, immunological and genetic properties of the pathogens indicating their advantages and limitations for selecting the appropriate technique to fulfill the requirements. Features: Presents techniques useful for detection, identification, quantification of microbial plant pathogens in plants, soil, and irrigation water from waterbodies. Highlights subversive activities of viruses, resulting in the breakdown of host defense systems. Discusses RNA silencing in infected plants by viruses and posttranscriptional gene silencing (PTGS) functioning as an endogenous mechanism in plants against virus infection. Presents information on methods of assessment of genetic variability and sensitivity of microbial plant pathogens to chemicals and adverse environmental conditions.

Soil has a versatile role in supporting the development of a wide range of organisms, including plants and microorganisms. Soilborne pathogens and root diseases are the primary limiting factor in many crops and tend to be very difficult to control. This first volume of a two-volume set introduces disease-causing microorganisms including oomycetes, fungi, bacteria, and viruses found in soils. It focuses on the biology, detection, and identification of soilborne bacterial, fungal, and viral plant pathogens. Volume two provides information on ecology and epidemiology of soilborne microbial plant pathogens and strategies applicable to manage diseases. Chapters cover exclusion and prevention strategies; improvement of host plant resistance; biological management; application of chemicals; and integration of disease management strategies.

POSTHARVEST PATHOGENS AND DISEASE MANAGEMENT
Postharvest diseases caused by microbial pathogens account for millions of dollars in losses of both durable and perishable produce products every year. Moreover, with consumers increasingly demanding minimally processed vegetables and fruits--which can be invaded by human pathogens--there is an imperative need for suitable protective measures to provide pathogen-free commodities that are free from, or contain only acceptable levels of, chemical residues.
Providing details of both conventional and modern molecular techniques applicable for the detection, identification, and differentiation of field and storage microbial pathogens, Postharvest Pathogens and Disease Management:
* Discusses diseases of both durables and perishables during transit and storage
* Provides a basic understanding of the effects of handling and storage practices as well as field conditions and product susceptibility on the development of postharvest diseases
* Reveals, as a cautionary note, the potential hazards of mycotoxins with carcinogenic properties that can contaminate fruits and vegetables
* Contains detailed information derived from elucidative evidence and disease data in order to explain the infection process and subsequent stages of disease development
* Helps readers to avoid conditions that favor disease incidence and spread
* Includes real life examples of disease management strategies to help readers develop effective disease management systems suitable for different ecosystems
* Emphasizes the importance of integrating several different effective methods in tandem, including the development of cultivars with resistance to postharvest diseases; the selection of suitable analytical methods; and the effective use of biocontrol agents and chemicals
* Presents protocols for numerous techniques and basic methods, making the book a distinctive and highly useful teaching and research tool
Postharvest Pathogens and Disease Management offers readers insight into the principles and methods of avoiding and managing postharvest diseases of fruit and vegetable products in an efficient, economical, and environmentally feasible manner, allowing producers to sell safer, higher-quality produce to the public and prevent the losses associated with postharvest disease.

Find out more about convenient immunoassays you can implement in your own research! From the Foreword, by M. S. Swaminathan, Chairman of the M. S. Swaminathan Research Foundation: The book provides remedies to the common maladies relating to quality and safety of dietary material. Professor Narayanasamy has compiled and presented with great clarity the latest information on all aspects relating to immunology in plant health and food safety. We owe Professor Narayanasamy a deep debt of gratitude for this labor of love in the cause of improving food and feed quality and safety. Immunology in Plant Health and Its Impact on Food Safety suggests cost-effective, simple, and sensitive immunological techniques to assess plant health and food safety for the production of desirable foods, feeds, and timbers. This book explores the structure and biochemical constituents of healthy plants and the abiotic and biotic stresses that can cause a marked reduction in quantity and quality of agricultural produce. Researchers, faculty members, and graduate scholars in plant pathology, microbiology, biochemistry, environmental sciences, and food technology will find this text useful for producing healthy plants while maintaining a pollution-free environment. In Immunology in Plant Health and Its Impact on Food Safety, methods to develop stress-resistant cultivars are discussed to enable you to select the most suitable strategies for maintaining production and quality without the use of chemicals. This valuable resource provides detailed instructions for employing immunoassays that are rapid, reproducible, and amenable for large-scale application in place of cumbersome and expensive methods currently in use. With this important tool, you will be able to plan and develop programs to obtain agricultural produce of high quality acceptable for human and animal consumption. With Immunology in Plant Health and Its Impact on Food Safety, you'll learn more about: agrosystems immunological reactions preparations of antisera immunodetection techniques plant-stress interactions genetic manipulations disease resistance and the production of disease-free plants mycotoxins chemical residues This essential guide provides you with access to a wide spectrum of information never before encompassed in a single book, saving you time and energy. Figures, photographs, and tables with appropriate data supply visual and factual support for the points discussed in the text. Immunology in Plant Health and Its Impact on Food Safety includes a large number of citations (over 1000) for further research and development in your chosen field of study.

Find out more about convenient immunoassays you can implement in your own research! From the Foreword, by M. S. Swaminathan, Chairman of the M. S. Swaminathan Research Foundation: The book provides remedies to the common maladies relating to quality and safety of dietary material. Professor Narayanasamy has compiled and presented with great clarity the latest information on all aspects relating to immunology in plant health and food safety. We owe Professor Narayanasamy a deep debt of gratitude for this labor of love in the cause of improving food and feed quality and safety. Immunology in Plant Health and Its Impact on Food Safety suggests cost-effective, simple, and sensitive immunological techniques to assess plant health and food safety for the production of desirable foods, feeds, and timbers. This book explores the structure and biochemical constituents of healthy plants and the abiotic and biotic stresses that can cause a marked reduction in quantity and quality of agricultural produce. Researchers, faculty members, and graduate scholars in plant pathology, microbiology, biochemistry, environmental sciences, and food technology will find this text useful for producing healthy plants while maintaining a pollution-free environment. In Immunology in Plant Health and Its Impact on Food Safety, methods to develop stress-resistant cultivars are discussed to enable you to select the most suitable strategies for maintaining production and quality without the use of chemicals. This valuable resource provides detailed instructions for employing immunoassays that are rapid, reproducible, and amenable for large-scale application in place of cumbersome and expensive methods currently in use. With this important tool, you will be able to plan and develop programs to obtain agricultural produce of high quality acceptable for human and animal consumption. With Immunology in Plant Health and Its Impact on Food Safety, you'll learn more about: agrosystems immunological reactions preparations of antisera immunodetection techniques plant-stress interactions genetic manipulations disease resistance and the production of disease-free plants mycotoxins chemical residues This essential guide provides you with access to a wide spectrum of information never before encompassed in a single book, saving you time and energy. Figures, photographs, and tables with appropriate data supply visual and factual support for the points discussed in the text. Immunology in Plant Health and Its Impact on Food Safety includes a large number of citations (over 1000) for further research and development in your chosen field of study.