To write a handbook of methods is surely to invite criticism, as has already been said several times. On the other hand, there is a great need for methodological manuals in all fields of science. It was therefore decided to compile this book, written in good faith to help scientists, teachers and students who will, it is hoped, use it and judge it good. To be useful to the reader, such a manual must provide a broad review of the methods available and describe them in sufficient detail to permit preliminary selection and judgement. It has to give - at least for selected methods - a suffi ciently detailed description of the equipment and procedure as to be to some extent self-contained. It must assume a critical standpoint as regards the theoretical basis of the methods, the significance of results, and their errors and limitations. It must also furnish examples, pertinent numerical tables, and very complete references. All this and much more is expected of a good manual of methods."

This volume contains a collection of papers on the anatomy, physiology and action of stomata by experts in the field, presented in a form suitable for students of botany and plant science, and related subjects such as agriculture, forestry, and horticulture, at the undergraduate and postgraduate level. Special care has been taken to ensure that the book can be used as a text for undergraduate courses in plant, environmental and cell physiology at a reasonably advanced level. It provides an up-to-date assessment of our knowledge of stomata and the articles include: the structure and development of stomata; the measurement of stomatal aperture and conductance; the ion exchanges and metabolism of guard cells; work on isolated guard-cell protoplasts; the responses of stomata to a wide range of stimuli, including light, carbon dioxide, humidity, water stress, pollutants and disease organisms; the behaviour of stomata in the field; and a discussion of stomatal control of transpiration and photosynthesis.