During its short 20 year history High Performance Liquid Chro matography (HPLC) has won itself a firm place amongst the instrumental methods of analysis. HPLC has caused a revolution in biological and pharmaceutical chemistry. Approximately two thirds of the publications on HPLC are concerned with problems from this area of life science. Biotechnology, where it is necessary to isolate substances from complicated mixtures, is likely to give further impetus to the dissemination of modern liquid chromatog raphy in columns, particularly on the preparative scale. This book presents, by means of examples, the application of HPLC to various fields, as well as fundamental discussions of chromatographic methods. The quality of the analytical result is decisively dependent on the qualities of the equipment employed (by Colin, Guiochon, and Martin). Especially the demands are discussed that are placed on the components of the instrument including those for data acquisition and processing. The section on "quantitative analy sis" (by ABhauer, Ullner) covers besides the principles also the problems of ensuring the quality of the data in detail. The basic problems arising by enlarging the sample size to preparative di mensions and the requirements put on the aparatus are discussed in the section on "preparative applications" (by Wehrli)."

Detectors for Liquid Chromatography Edited by Edward S. Yeung Written by an expert in the field, this comprehensive guide explains the basic principles behind detector response instrumentation and selected applications. Early chapters cover absorption detectors for high performance liquid chromatography, FTIR detection, indirect absorbance detectors, fluorometric detection, and polarimetric detectors. Coverage continues with detection based on electrical and electrochemical measurements, mass spectrometry as an online detector for HPLC, and miscellaneous methods. 1986 0 471-82169-1 366 pp. Small Bore High Performance Liquid Chromatography Edited by Raymond R. W. Scott A state-of-the-art guide that demonstrates how to design, construct, and pack optimized small bore columns, the center of any chromatography system. Case examples show the use of these columns for high resolution, very fast analysis, and special methods for molecular weight determinations. Applications from a wide range of industrial and forensic analyses aid in developing sophistication in a number of useful techniques. The book provides essential information on topics such as calculating the minimum column radius, detectors, and molecular diffusion. Includes 92 illustrations and 14 tables to enhance explanations of microbore HPLC methods. 1984 0 471-80052-X 271 pp. Reversed-Phase High Performance Liquid Chromatography: Theory, Practice, and Biomedical Applications Ante M. Krstulovic and Phyllis R. Brown Reversed-phase liquid chromatography has increased tremendously in popularity over the past ten years. Estimates show that more than 80 per cent of all HPLC separations are performed using this technique. This book covers both theoretical aspects of RPLC and practical information needed in diverse areas of research; it also contains a review of the RPLC applications in the biomedical/biochemical field, with references and collateral readings. Material is presented in a practical, problem-solving manner and should be immensely useful in theoretical aspects of RPLC and all areas of scientific research, particularly the biomedical/biochemical field, where RPLC has made its largest impact. 1982 0 471-05369-4 296 pp.

This collection features five peer-reviewed literature reviews on nematode pests in agriculture. The first chapter assesses the value and impact of using integrated pest management (IPM) to combat nematode pests. It summarises the basic biology and morphology of the most economically-important nematode pests and reviews cultural, biological and chemical methods of control. The second chapter discusses advances in understanding plant root response to nematode attack. It highlights how nematodes form feeding structures in host roots and how this behaviour has allowed plants to evolve new mechanisms of resistance that target this structure with a toxic response. The third chapter discusses key nematode pests of wheat, including cereal cyst, root-knot and root-lesion nematodes. It features research on best practice in detecting and managing these pests effectively. The fourth chapter summarises what we know about soil and plant nematodes damaging maize, including lesion, root-knot and vermiform nematodes. It reviews research on characteristics, identification and effects which provide the foundation for effective control. The final chapter describes the characteristics and control of key nematode pests of potatoes: potato cyst nematodes, root knot nematodes, root lesion nematode, potato rot and stem nematodes as well as virus vector nematodes.