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Practice of High Performance Liquid Chromatography - Applications, Equipment and Quantitative Analysis (Paperback, Softcover reprint of the original 1st ed. 1986)
Loot Price: R1,541
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Practice of High Performance Liquid Chromatography - Applications, Equipment and Quantitative Analysis (Paperback, Softcover reprint of the original 1st ed. 1986)
Series: Chemical Laboratory Practice
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During its short 20 year history High Performance Liquid Chro
matography (HPLC) has won itself a firm place amongst the
instrumental methods of analysis. HPLC has caused a revolution in
biological and pharmaceutical chemistry. Approximately two thirds
of the publications on HPLC are concerned with problems from this
area of life science. Biotechnology, where it is necessary to
isolate substances from complicated mixtures, is likely to give
further impetus to the dissemination of modern liquid chromatog
raphy in columns, particularly on the preparative scale. This book
presents, by means of examples, the application of HPLC to various
fields, as well as fundamental discussions of chromatographic
methods. The quality of the analytical result is decisively
dependent on the qualities of the equipment employed (by Colin,
Guiochon, and Martin). Especially the demands are discussed that
are placed on the components of the instrument including those for
data acquisition and processing. The section on "quantitative analy
sis" (by ABhauer, Ullner) covers besides the principles also the
problems of ensuring the quality of the data in detail. The basic
problems arising by enlarging the sample size to preparative di
mensions and the requirements put on the aparatus are discussed in
the section on "preparative applications" (by Wehrli)."
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