This volume aims to describe a variety of techniques that reflects the wide range of research currently performed in the field of coronavirology, and begins with an overview of current understandings of coronavirus replication and pathogenesis to introduce specialists and non-specialists to the field. The rest of the book is divided into several sections of chapters beginning with those that describe identification, diagnosis, and study of the evolution of coronaviruses. The next few chapters discuss the preparation of cells and organ cultures useful in propagating coronaviruses and titration techniques, as well as techniques for analyzing virus functions that require purification of the viruses. The next chapters describe two commonly used reverse genetics techniques for coronaviruses, and techniques detailing identification of cellular receptors, binding profiles of viral attachment proteins, and virus-cell fusion. The final chapters cover a broad spectrum of techniques to identify virus-host protein-protein interactions, confirm the functional role of these proteins in virus replication, study host cell responses through genome-wide or pathway-specific approaches, and visualize virus replication complexes. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Authoritative and practical, Coronaviruses: Methods and Protocols appeals to a wide variety of scientists because it highlights techniques that are currently used in the coronovirology field, while also discussing practices applicable to other virology fields.

'Riveting... Everyone should read it' Observer 'Nothing short of sudden death will distract you from The Jigsaw Man' Independent 'Compelling... Fascinating... Britton has done hugely important work that saves lives' Sunday Times ___________________________________________ The award-winning true crime classic. Forensic psychologist Paul Britton asks himself four questions when he is faced with a crime scene: what happened: who is the victim: how was it done, and why? Only when he has the answers to these questions can he address the fifth: who is responsible? What he searches for at the crime scene are not frinerprints, fibres or bloodstains - he looks for the 'mind trace' left behind by those responsible: the psychological characteristics that can help the police to identify and understand the nature of the perpetrator. The Jigsaw Man is not only a detective story involving some of the most high-profile cases of recent years, but also a journey of discovery into the darkest recesses of the human mind to confront the question 'Where does crime come from?'