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Books > Science & Mathematics > Biology, life sciences > Microbiology (non-medical)
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Coronaviruses - Methods and Protocols (Hardcover, 2015 ed.)
Loot Price: R4,814
Discovery Miles 48 140
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Coronaviruses - Methods and Protocols (Hardcover, 2015 ed.)
Series: Methods in Molecular Biology, 1282
Expected to ship within 12 - 17 working days
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This volume aims to describe a variety of techniques that reflects
the wide range of research currently performed in the field of
coronavirology, and begins with an overview of current
understandings of coronavirus replication and pathogenesis to
introduce specialists and non-specialists to the field. The rest of
the book is divided into several sections of chapters beginning
with those that describe identification, diagnosis, and study of
the evolution of coronaviruses. The next few chapters discuss the
preparation of cells and organ cultures useful in propagating
coronaviruses and titration techniques, as well as techniques for
analyzing virus functions that require purification of the viruses.
The next chapters describe two commonly used reverse genetics
techniques for coronaviruses, and techniques detailing
identification of cellular receptors, binding profiles of viral
attachment proteins, and virus-cell fusion. The final chapters
cover a broad spectrum of techniques to identify virus-host
protein-protein interactions, confirm the functional role of these
proteins in virus replication, study host cell responses through
genome-wide or pathway-specific approaches, and visualize virus
replication complexes. Written in the highly successful Methods in
Molecular Biology series format, the chapters include the kind of
detailed description and implementation advice that is crucial for
getting optimal results in the laboratory. Authoritative and
practical, Coronaviruses: Methods and Protocols appeals to a wide
variety of scientists because it highlights techniques that are
currently used in the coronovirology field, while also discussing
practices applicable to other virology fields.
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