PCR, developed at Cetus Corporation/USA by Henry A. Erlich, Kary Mullis and Randall K. Saiki, is a very simple method for amplifying nucleic acids in vitro. The realization of this idea bases on the repetition of a set of three different temperatures and yields an increase of the target structure up to a factor of 106 to 1012. Therefore, this technique is predisposed for safe analysis and characterization of DNA and RNA sequences of interest, even where the starting amount of material is enormously small. Because of its sensitivity, speed and versatility this method is particularly suitable for investigations of oncogenes, tumor associated translocations, retroviral sequences, lymphokines and mainly the broad field of degenerative and inflammatory diseases of nervous system. PCR seems to be the technique which could overcome the two most important problems in that field: very small amount of material combined with the necessity of rapid diagnostic procedures in inflammatory infections. "PCR topics" will give an actual overview of basic and applied research fields on usage of polymerase chain reaction. All contributions to this book have been presented at an international congress on "Usage of Polymerase chain reaction in genetic and infectious diseases" which took place in june 1990 in Berlin. The editors wish to thank all participants for their contributions. We offer our thanks and gratitude to our coworkers and especially to our technical assistents Barbara Trampenau, Mirjana Wiirdemann and Hannelore Leonhard.

avec la collaboration/in Zusammenarbeit mit Jean-Louis Besson, Segolene Le Men, Peter W. Marx et/und Clara Royer Pionnier des temps presents et a venir, Max Reinhardt (1873-1943) fait du theatre le vecteur meme de la modernite et de ses bouleversements de frontieres. Il procede ainsi a des decloisonnements multiples et a une recherche constante de nouveaux espaces. A la mesure de cette proposition, par nature experimentale, polymorphe et cosmopolite, le present volume s'emploie a multiplier les circulations entre les disciplines et les territoires de la recherche. Par la rencontre des conservateurs d'archives et des chercheurs, par le dialogue des specialistes avec les representants des aires traversees, il devient possible de composer un atlas artistique et culturel de cette epoque et de prendre la mesure de la posterite de Max Reinhardt jusqu'a nos jours. Als Pionier seiner Zeit und der Zukunft sieht Max Reinhardt (1873-1943) im Theater einen Trager der Moderne und ihrer vielfaltigen Umwalzungen. Auf der Suche nach neuen Schaffensraumen unterzieht er die Buhne einem radikalen Entgrenzungsprozess. Gemass dieser von Grund auf experimentellen, polymorphen und kosmopolitischen Geste basiert diese Publikation auf Zirkulationen zwischen den wissenschaftlichen Disziplinen und Territorien. Durch die Kollaboration der Archivare und der Forscher, durch den Dialog zwischen den Max Reinhardt-Spezialisten und den Vertretern der zahlreichen Wirkungsgebiete entsteht ein kunstlerischer und kultureller Atlas von dieser Epoche und von Max Reinhardts Erbe bis heute.

An essay examining various characters in Jane Austen's Pride and Prejudice, and why Faith, Hope and Love are the key Christian virtues being explored internally by the author.

How far from your native country would you travel to find a place to live in freedom? What if it meant leaving behind everything and everyone you ever loved, if only because of a slim chance that a better life might exist someplace else? This is the story of a thirty-two year-old Italian stonecutter who had such a dream, as well as the faith and the courage to make it become a reality for himself and his family. Pasquale DeVito was a man with no money, education, or connections. One day while walking in his village he saw a poster; and the poster slowly turned into a mighty dream. That dream would take him across an ocean, to a land desperate for strong, hard-working men to complete the building of a modern new nation. This is his story, the story of his descendants, and the story of America.

Fate...Free Will...Destiny...Predestination. What do all these have in common? Do human beings really have free will, or does God and Nature have a say in the matter? This novel answers those questions for one man in particular. Richard Desmond lived at the end of the 20th Century and was both an accountant and a poet. His life's portrait was composed of a series of choices; and whether they were good or bad, readers must decide for themselves.

The 19th century ushered in an unprecedented boom in technology, the unification of European nations, the building of global empires and stabilization of the middle classes. The theatre of the era reflected these significant developments as well as helped to catalyse them. Populist theatre and purposebuilt playhouses flourished in the ever-growing urban and cosmopolitan centres of Europe and in expanding global networks. This volume provides a comprehensive and interdisciplinary overview of the cultural history of theatre from 1800 to 1920. Highly illustrated with 51 images, the ten chapters each take a different theme as their focus: institutional frameworks; social functions; sexuality and gender; the environment of theatre; circulation; interpretations; communities of production; repertoire and genres; technologies of performance; and knowledge transmission.

Friedrich Adolph Krummacher war ein evangelischer Theologe und zugleich ein Bestsellerautor der Goethezeit. Eine Vielzahl von ausserordentlichen Dokumenten ermoglicht es, ein ganz nahes und lebendiges Bild von ihm zu gewinnen und mittels seiner Bekanntschaft in eine Zeit einzutauchen, in der uns manches fremd und vorgestrig, vieles aber auch uberraschend nah und zuganglich erscheint. Das Buch konzentriert sich dabei auf die Kettwiger Jahre, 1807 - 1812; eine historische Ausnahmesituation, die Franzosenzeit" unter Napoleon. Wie erlebt der Dichter, Pfarrer, Freund und Familienvater diese Zeit? In seinen Werken, noch mehr aber in seinen zahlreichen Briefen und zeitgenossischen Urteilen, zeigt sich ein ausgesprochen liebenswerter, humorvoller und geistreicher Mensch. Seinen vielfaltigen Spuren und damit der Lebensart von vor 200 Jahren nachzugehen, macht Zeit- und Kulturgeschichte auf faszinierende Weise erfahrbar. Mit zahlreichen Originaltexten und Noten