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PCR Topics - Usage of Polymerase Chain Reaction in Genetic and Infectious Diseases (Paperback)
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PCR Topics - Usage of Polymerase Chain Reaction in Genetic and Infectious Diseases (Paperback)
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PCR, developed at Cetus Corporation/USA by Henry A. Erlich, Kary
Mullis and Randall K. Saiki, is a very simple method for amplifying
nucleic acids in vitro. The realization of this idea bases on the
repetition of a set of three different temperatures and yields an
increase of the target structure up to a factor of 106 to 1012.
Therefore, this technique is predisposed for safe analysis and
characterization of DNA and RNA sequences of interest, even where
the starting amount of material is enormously small. Because of its
sensitivity, speed and versatility this method is particularly
suitable for investigations of oncogenes, tumor associated
translocations, retroviral sequences, lymphokines and mainly the
broad field of degenerative and inflammatory diseases of nervous
system. PCR seems to be the technique which could overcome the two
most important problems in that field: very small amount of
material combined with the necessity of rapid diagnostic procedures
in inflammatory infections. "PCR topics" will give an actual
overview of basic and applied research fields on usage of
polymerase chain reaction. All contributions to this book have been
presented at an international congress on "Usage of Polymerase
chain reaction in genetic and infectious diseases" which took place
in june 1990 in Berlin. The editors wish to thank all participants
for their contributions. We offer our thanks and gratitude to our
coworkers and especially to our technical assistents Barbara
Trampenau, Mirjana Wiirdemann and Hannelore Leonhard.
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