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In Peptide Modifications to Increase Metabolic Stability and
Activity, expert researchers in the field provide summarized
methods for preparation, purification of modified peptides, and
assessment of their biochemical activities. These methods and
protocols include preparation of conformationally constrained
peptides, modification of peptide bonds, introduction of
nonproteinogenic amino acids, and alteration of peptides' physical
and biological properties by modification of the amino acid side
chains and/or terminal residues. With additional chapter that
describes new experimental approach for the detection of exogenous
peptides within living cells using peptides labeled with heavy
isotopes and confocal Raman microscopy. Written in the highly
successful Methods in Molecular Biology series format, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and practical, Peptide
Modifications to Increase Metabolic Stability and Activity seeks to
provide scientists with alternative approaches to peptide
modification that many researchers may find applicable to their
specific research requirements.
In Peptide Modifications to Increase Metabolic Stability and
Activity, expert researchers in the field provide summarized
methods for preparation, purification of modified peptides, and
assessment of their biochemical activities. These methods and
protocols include preparation of conformationally constrained
peptides, modification of peptide bonds, introduction of
nonproteinogenic amino acids, and alteration of peptides' physical
and biological properties by modification of the amino acid side
chains and/or terminal residues. With additional chapter that
describes new experimental approach for the detection of exogenous
peptides within living cells using peptides labeled with heavy
isotopes and confocal Raman microscopy. Written in the highly
successful Methods in Molecular Biology series format, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and practical, Peptide
Modifications to Increase Metabolic Stability and Activity seeks to
provide scientists with alternative approaches to peptide
modification that many researchers may find applicable to their
specific research requirements.
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