Plant stresses are serious threats to the sustainability of crop yields accounting for more crop productivity losses than any other factor in rainfed agriculture. Post-harvest losses mean surplus crops do not reach market, affecting the livelihoods of farming families, and too often these families are left with no other option than to eat contaminated stored food. These constraints impact the food security of these farming families as well as the communities and countries in which they live. This book is the demonstration of a clear synergistic effect of stresses, an effect that was unexpectedly as important as either stress applied alone. This book will add to our current knowledge of abiotic stress response in plants and will provide the groundwork necessary to build future strategies for crop enhancement. The fundamental principles that underpin all biotechnology are explained and a full range of examples discussed to show how these principles are applied; from starting substrate to final product. It will be beneficial to both plant breeders and molecular biologists, because it combines the topics of physiology, tolerance genes, and breeding methods. When these topics are presented together, it is easy to compare all aspects of tolerance mechanisms and breeding methods for abiotic stresses. These comparisons are useful to understand which pathways or which genes are important for rendering more tolerance to a certain abiotic stress, and to bring forward new ideas for improving the tolerance. Features *Cover both plant biotic and abiotic stresses *Important factors in managing crops for water stress conditions *Substantially increase the sustainable productivity of smallholder farmers in developing countries *Genetic and biochemical approaches - if those approaches constitute a substantial improvement on current practices.

RNAi technology are used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. This book reviews the selective and robust effect of RNAi on gene expression which makes it a valuable research tool, both in cell culture and in living organisms because synthetic dsRNA introduced into cells can induce suppression of specific genes of interest. This book will introduce new technology in the study of RNA interference in microorganisms, plants and animals.

Geminivirus: Detection, Diagnosis and Management focuses on the latest techniques for managing diseases caused by these circular, single-stranded (ss) DNA genomes. The most significant impact of plant diseases in host populations is often caused by emerging diseases, whose incidence in a plant host is increasing as a result of long-term changes in their underlying epidemiology. Genetic changes in pathogen and host populations, as well as changes in host ecology and environment, are major factors contributing to disease emergence. Understanding plant virus evolution is crucial for modeling the within-host and between-host dynamics and genetics of virus populations. The book presents a comprehensive review of how these viruses develop, including contributing factors such as population bottlenecks during cell-to-cell movement, systemic colonization, or between-host transmission by different procedures. Presented in five sections-Detection and Diagnosis, Emergence and Diversity, Vector and Transmission, Virus-Host Interaction, and Disease Management, the book includes host range determinant and virulence factors involved in pathogenesis, virus-vector interactions during acquisition, retention, and transmission and evaluating management strategies to control Geminivirus. The book is an essential reference for students and researchers interested in plant virology, particularly begomoviruses, geminiviruses, and vector transmission biology. Introduces identification and characterization of geminiviruses that infect agricultural crops, their wild relatives, and weed hosts Discusses recombination and reassortment mechanisms influencing viral genetic diversity, virulence, and vector transmission Explores the origin, evolution, and bottlenecks of Geminiviruses

RNAi technology are used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. This book reviews the selective and robust effect of RNAi on gene expression which makes it a valuable research tool, both in cell culture and in living organisms because synthetic dsRNA introduced into cells can induce suppression of specific genes of interest. This book will introduce new technology in the study of RNA interference in microorganisms, plants and animals.

"Plant Virus-Host Interaction" contains cutting-edge research in plant molecular virology, including pathogenic viroids and transport by insect vectors, interference with transmission to control viruses, and synergism, with pivotal coverage of RNA silencing and the counter-defensive strategies used by viruses to overcome the silencing response in plants.

With a clear focus on plant virus evolution, including quantitative and population genetics, "Plant Virus-Host Interaction" provides insights on the major factors favoring disease emergence, such as genetic change in pathogen and host populations and changes in host ecology and environment. The book also examines socioeconomic implications of widespread plant viral agents. Contributions from leading experts around the globe provide varied perspectives, while comprehensive coverage ensures a complete look at this exciting field.
Covers the emergence of new viral diseasesProvides molecular approaches for virus-host interactionHighlights RNA silencing and counter-defensive strategiesDiscusses socioeconomic implications of viral spread and mitigation techniques

Bioinformatics in Agriculture: Next Generation Sequencing Era is a comprehensive volume presenting an integrated research and development approach to the practical application of genomics to improve agricultural crops. Exploring both the theoretical and applied aspects of computational biology, and focusing on the innovation processes, the book highlights the increased productivity of a translational approach. Presented in four sections and including insights from experts from around the world, the book includes: Section I: Bioinformatics and Next Generation Sequencing Technologies; Section II: Omics Application; Section III: Data mining and Markers Discovery; Section IV: Artificial Intelligence and Agribots. Bioinformatics in Agriculture: Next Generation Sequencing Era explores deep sequencing, NGS, genomic, transcriptome analysis and multiplexing, highlighting practices forreducing time, cost, and effort for the analysis of gene as they are pooled, and sequenced. Readers will gain real-world information on computational biology, genomics, applied data mining, machine learning, and artificial intelligence. This book serves as a complete package for advanced undergraduate students, researchers, and scientists with an interest in bioinformatics.

Plant Virus-Host Interaction: Molecular Approaches and Viral Evolution, Second Edition, provides comprehensive coverage of molecular approaches for virus-host interaction. The book contains cutting-edge research in plant molecular virology, including pathogenic viroids and transport by insect vectors, interference with transmission to control viruses, synergism with pivotal coverage of RNA silencing, and the counter-defensive strategies used by viruses to overcome the silencing response in plants. This new edition introduces new, emerging proteins involved in host-virus interactions and provides in-depth coverage of plant virus genes' interactions with host, localization and expression. With contributions from leading experts, this is a comprehensive reference for plant virologists, molecular biologists and others interested in characterization of plant viruses and disease management.

Botany is a forerunning field of study that could address the foremost disputes facing humanity in the 21st century; coupled with the development of new tools and techniques, it could aid in finding solutions to diagnose these unsolved questions. The present book summarises the recent technological advances of plant science. This is a succinct, up-to-date, and relevant compilation based on the techniques used in plant science; this book embodies the recent knowledge of advanced techniques frequently being used from last few decades. There are twelve chapters summarised systematically that demonstrate: the suitability of Laser Produced Plasma Spectroscopy; potential of NMR spectroscopy in plant metabolomics; recent developments and applications of novel analytical techniques for the analysis of plant materials; chlorophyll as a fluorescence analysis and a technique to explore the photochemistry of photosystem II; drought stress and metabolomics in plants; heat shock on Bipolaris sorokiniana in cereal crops; salt stress on crops plants and their mitigation strategies; plant cell death by rumen-induced stress on plant in ruminant herbivores; the role of diazotrophs in maintaining plant health in agricultural fields; population biology of Rhizoctonia solani AG-1 IA from India; and biofilms as a home for microorganisms and genetically modified organism. This book will be helpful for researchers, academicians and students working on the related fields.

Biotechnology has proven to be one of the more fruitful technological approaches in science today; being both very powerful, and able to generate valuable intellectual property. This book aims to present examples in the applications of molecular biology and genetic engineering. This book shows how areas as diverse as agrofood technology, fine chemistry, detergents, bioremediation and biosensors are receiving significant contributions from biotechnology approaches. The fundamental principles that underpin all biotechnologies are explained and a full range of examples are discussed to show how these principles are applied; from starting substrates to the final product. The challenges on these aspects and methodologies inspired us to edit this book.