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Taro leaf blight caused by Phytophthora colocasiae is a major
constraint to taro cultivation. To manage taro leaf blight and
design breeding programme, it is crucial to recognize the genetic
diversity of taro and P. colocasiae. We studied genetic structure
of taro and P. colocasiae in India. Biochemical alterations in taro
plants infected by the P. colocasiae were studied and changes in
peroxidase, -1,3-glucanase, L-phenylalanine ammonia-lyase, total
phenol and total sugar were evaluated. We employed suppressive
subtractive hybridization, high through put DNA sequencing and
bioinformatics to identify the defense related genes in taro
induced by P. colocasiae infection. Two putative resistance genes
and a transcription factor were identified among the upregulated
sequences. Our study illustrated that identified genes will be
useful to engineer disease protection in taro."
Leaf blight of taro, caused by Phytophthora colocasiae Raciborski,
is the most destructive disease of Colocasia esculenta.The disease
has severely constrained taro production. Based on disease severity
its highly solicited to develop disease prevention method. The use
of disease-free planting material is important in halting the
spread of P. colocasiae from nurseries to fields and also highly
relevant to limiting the spread of it across the globe.In view of
that we have developed Nested PCR-Primers for rapid and sensitive
detection of Phytophthora colocasiae in infected planting material
and infested soil. We have further aimed at investigating the role
of BAP(synthetic cytokinin), pathogen derived elicitor protein in
the interaction of P. colocasiae and its host plant taro, to
explore its potential in controlling taro leaf blight disease.Our
investigation shows that transgenic taro plants expressing eiher an
elicitor protein or increased cytokinin level could be useful to
engineer broad disease protection in taro plant against
Phytophthora leaf blight.
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