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First Published in 2005. Routledge is an imprint of Taylor &
Francis, an informa company.
As with many other languages, Mandarin Chinese exhibits a rich
variety of ways in expressing the arguments of the predicator in a
sentence. Unlike other languages, such variation is typically
devoid of any formal marking. Previous attempts in explaining such
phenomena usually focus on the syntax as an explanatory tool. The
main purpose of this book is to argue that many of such argument
structure phenomena are better accounted for by recourse to
enriched representations in lexical semantics. Drawing insights
from conceptual semantics, cognitive semantics, Generative Lexicon,
construction grammar and formal syntax, this book constitutes the
first attempt at a comprehensive account of lexical semantic issues
in Mandarin Chinese.
Molecular Medical Microbiology, Third Edition presents the latest
release in what is considered to be the first book to synthesize
new developments in both molecular and clinical research. The
molecular age has brought about dramatic changes in medical
microbiology, along with great leaps in our understanding of the
mechanisms of infectious disease. This third edition is completely
updated, reviewed and expanded, providing a timely and helpful
update for microbiologists, students and clinicians in the era of
increasing use of molecular techniques, changing epidemiology and
prevalence, and increasing resistance of many pathogenic bacteria.
Written by experts in the field, chapters include cutting-edge
information and clinical overviews for each major bacterial group,
along with the latest updates on vaccine development, molecular
technology and diagnostic technology.
This book provides state-of-the-art information on gene
essentiality screenings in a wide variety of organisms, i.e.
screening for protein-coding genes and other genomic elements that
are required by an organism to survive under specific conditions.
With a focus on the two techniques that have revolutionized the
field, the collection begins with chapters employing
CRISPR/Cas9-based approaches followed by Tn-seq-based approaches,
but later chapters also delve into other techniques for exploring
essential genes, such as bioinformatics methods. Written for the
highly successful Methods in Molecular Biology series, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Comprehensive and authoritative, Essential
Genes and Genomes: Methods and Protocols is an ideal guide for
researchers attempting to strip genetics down to its fundamentals.
This book provides state-of-the-art information on gene
essentiality screenings in a wide variety of organisms, i.e.
screening for protein-coding genes and other genomic elements that
are required by an organism to survive under specific conditions.
With a focus on the two techniques that have revolutionized the
field, the collection begins with chapters employing
CRISPR/Cas9-based approaches followed by Tn-seq-based approaches,
but later chapters also delve into other techniques for exploring
essential genes, such as bioinformatics methods. Written for the
highly successful Methods in Molecular Biology series, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Comprehensive and authoritative, Essential
Genes and Genomes: Methods and Protocols is an ideal guide for
researchers attempting to strip genetics down to its fundamentals.
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