Among different pectinases, pectin lyase seems to be the only pectic enzyme capable of breaking down pectin with a high degree of esterification. Trichoderma harziamun is excellent pectinases producer, therefore different isolates were screened by quantitative and qualitative methods. Screening results showed that two isolates showed maximum pectin lyase. Different fermentation conditions like fermentation rate, inoculum size, concentration of peptone, concentration of yeast extract, pH and tween -80 were optimized by different experiments for production of pectin lyase by T. harzianum isolates. Pectin lyase was partially purified by ammonium sulphate precipitation. The fraction, which contained a large amount of pectin lyase was dialyased against 0.2 M phosphate buffer at pH 5.5 for 12 hours and used for SDS-PAGE and characterization of pectin lyase. The partial purified pectin lyase (PL) from T140 and T 325 isolates had a molecular weight of 22 kDa. Purified enzyme form isolates were also characterized at different pH temperature and substrate range. Results were discussed on the basis of to statistical analysis of variance (ANOVA) under Complete Randomized Design (CRD).

Biosorption is being demonstrated as a useful alternative to conventional systems for the removal of toxic metals from industrial effluents. Metal affinity to the biomass can be increase by pre treating the biomass with different chemicals. Current study was deigned to assess the Ni(II) and CCu(II) removal capability of two microfungi viz. Aspergillus niger and Rhizopus arrhizus. Biomass of both fungal species was pretreated with alkalies, acids and detergents. For the successful application of biosorption crucial parameters like influence of initial metal ion concentration and desorption/reutilization were also performed. To check the practical potential of adsorbents, metal uptake capability of test fungal biosorbents was assessed with real industrial effluents. This book is useful for those who are working on treatment of wastewater using fungal biomass.