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Purification of Pectin Lyase by Trichoderma harzianum (Paperback)
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Purification of Pectin Lyase by Trichoderma harzianum (Paperback)
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Among different pectinases, pectin lyase seems to be the only
pectic enzyme capable of breaking down pectin with a high degree of
esterification. Trichoderma harziamun is excellent pectinases
producer, therefore different isolates were screened by
quantitative and qualitative methods. Screening results showed that
two isolates showed maximum pectin lyase. Different fermentation
conditions like fermentation rate, inoculum size, concentration of
peptone, concentration of yeast extract, pH and tween -80 were
optimized by different experiments for production of pectin lyase
by T. harzianum isolates. Pectin lyase was partially purified by
ammonium sulphate precipitation. The fraction, which contained a
large amount of pectin lyase was dialyased against 0.2 M phosphate
buffer at pH 5.5 for 12 hours and used for SDS-PAGE and
characterization of pectin lyase. The partial purified pectin lyase
(PL) from T140 and T 325 isolates had a molecular weight of 22 kDa.
Purified enzyme form isolates were also characterized at different
pH temperature and substrate range. Results were discussed on the
basis of to statistical analysis of variance (ANOVA) under Complete
Randomized Design (CRD).
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