There is a growing need in both industrial and academic research to obtain accurate quantitative results from continuous wave (CW) electron paramagnetic resonance (EPR) experiments. This book describes various sample-related, instrument-related and software-related aspects of obtaining quantitative results from EPR expe- ments. Some speci?c items to be discussed include: selection of a reference standard, resonator considerations (Q, B ,B ), power saturation, sample position- 1 m ing, and ?nally, the blending of all the factors together to provide a calculation model for obtaining an accurate spin concentration of a sample. This book might, at ?rst glance, appear to be a step back from some of the more advanced pulsed methods discussed in recent EPR texts, but actually quantitative "routine CW EPR" is a challenging technique, and requires a thorough understa- ing of the spectrometer and the spin system. Quantitation of CW EPR can be subdivided into two main categories: (1) intensity and (2) magnetic ?eld/mic- wave frequency measurement. Intensity is important for spin counting. Both re- tive intensity quantitation of EPR samples and their absolute spin concentration of samples are often of interest. This information is important for kinetics, mechanism elucidation, and commercial applications where EPR serves as a detection system for free radicals produced in an industrial process. It is also important for the study of magnetic properties. Magnetic ?eld/microwave frequency is important for g and nuclear hyper?ne coupling measurements that re?ect the electronic structure of the radicals or metal ions.

Distance measurements in biological systems by EPR The foundation for understanding function and dynamics of biological systems is knowledge of their structure. Many experimental methodologies are used for determination of structure, each with special utility. Volumes in this series on Biological Magnetic Resonance emphasize the methods that involve magnetic resonance. This volume seeks to provide a critical evaluation of EPR methods for determining the distances between two unpaired electrons. The editors invited the authors to make this a very practical book, with specific numerical examples of how experimental data is worked up to produce a distance estimate, and realistic assessments of uncertainties and of the range of applicability, along with examples of the power of the technique to answer biological problems. The first chapter is an overview, by two of the editors, of EPR methods to determine distances, with a focus on the range of applicability. The next chapter, also by the Batons, reviews what is known about electron spin relaxation times that are needed in estimating distances between spins or in selecting appropriate temperatures for particular experiments. Albert Beth and Eric Hustedt describe the information about spin-spin interaction that one can obtain by simulating CW EPR line shapes of nitroxyl radicals. The information in fluid solution CW EPR spectra of dual-spin labeled proteins is illustrated by Hassane Mchaourab and Eduardo Perozo.

Biomedical EPR Part B focuses on applications of EPR techniques and instrumentation, with applications to dynamics. The book celebrates the 70th birthday of Prof. James S. Hyde, Medical College of Wisconsin, and his contributions to this field. Chapters are written to provide introductory material for new-comers to the field that lead into up-to-date reviews that provide perspective on the wide range of questions that can be addressed by EPR.

Key Features:
EPR Techniques including Saturation Recovery, ENDOR, ELDOR, and Saturation Transfer

Instrumentation Innovations including Loop Gap Resonators, Rapid Mixing, and Time Locked Sub-Sampling

Motion in Biological Membranes

Applications to Structure Determination in Proteins

Discussion of Trends in EPR Technology and Prognosis for the Future "

Biomedical EPR - Part A focuses on applications of EPR spectroscopy in the areas of free radicals, metals, medicine, and physiology. The book celebrates the 70th birthday of Prof. James S. Hyde, Medical College of Wisconsin, and his contributions to this field. Chapters are written to provide introductory material for new-comers to the field which lead into up-to-date reviews that provide perspective on the wide range of questions that can be addressed by EPR.

Key Features:
Free Radicals in Medicine

Radicals in vivo and in Model Systems, and their Study by Spin Trapping

In vivo EPR, including Oximetry and Imaging

Time Domain EPR at Radio Frequencies

EPR of Copper Complexes: Motion and Frequency Dependence

Time Domain EPR and Electron Spin Echo Envelope Modulation

There is a growing need in both industrial and academic research to obtain accurate quantitative results from continuous wave (CW) electron paramagnetic resonance (EPR) experiments. This book describes various sample-related, instrument-related and software-related aspects of obtaining quantitative results from EPR expe- ments. Some speci?c items to be discussed include: selection of a reference standard, resonator considerations (Q, B, B ), power saturation, sample position- 1 m ing, and ?nally, the blending of all the factors together to provide a calculation model for obtaining an accurate spin concentration of a sample. This book might, at ?rst glance, appear to be a step back from some of the more advanced pulsed methods discussed in recent EPR texts, but actually quantitative "routine CW EPR" is a challenging technique, and requires a thorough understa- ing of the spectrometer and the spin system. Quantitation of CW EPR can be subdivided into two main categories: (1) intensity and (2) magnetic ?eld/mic- wave frequency measurement. Intensity is important for spin counting. Both re- tive intensity quantitation of EPR samples and their absolute spin concentration of samples are often of interest. This information is important for kinetics, mechanism elucidation, and commercial applications where EPR serves as a detection system for free radicals produced in an industrial process. It is also important for the study of magnetic properties. Magnetic ?eld/microwave frequency is important for g and nuclear hyper?ne coupling measurements that re?ect the electronic structure of the radicals or metal ions.

Biomedical EPR a " Part B focuses on applications of EPR techniques and instrumentation, with applications to dynamics. The book celebrates the 70th birthday of Prof. James S. Hyde, Medical College of Wisconsin, and his contributions to this field. Chapters are written to provide introductory material for new-comers to the field that lead into up-to-date reviews that provide perspective on the wide range of questions that can be addressed by EPR.

Key Features:
EPR Techniques including Saturation Recovery, ENDOR, ELDOR, and Saturation Transfer

Instrumentation Innovations including Loop Gap Resonators, Rapid Mixing, and Time Locked Sub-Sampling

Motion in Biological Membranes

Applications to Structure Determination in Proteins

Discussion of Trends in EPR Technology and Prognosis for the Future

Biomedical EPR - Part A focuses on applications of EPR spectroscopy in the areas of free radicals, metals, medicine, and physiology. The book celebrates the 70th birthday of Prof. James S. Hyde, Medical College of Wisconsin, and his contributions to this field. Chapters are written to provide introductory material for new-comers to the field which lead into up-to-date reviews that provide perspective on the wide range of questions that can be addressed by EPR.

Key Features:
Free Radicals in Medicine

Radicals in vivo and in Model Systems, and their Study by Spin Trapping

In vivo EPR, including Oximetry and Imaging

Time Domain EPR at Radio Frequencies

EPR of Copper Complexes: Motion and Frequency Dependence

Time Domain EPR and Electron Spin Echo Envelope Modulation

Distance measurements in biological systems by EPR The foundation for understanding function and dynamics of biological systems is knowledge of their structure. Many experimental methodologies are used for determination of structure, each with special utility. Volumes in this series on Biological Magnetic Resonance emphasize the methods that involve magnetic resonance. This volume seeks to provide a critical evaluation of EPR methods for determining the distances between two unpaired electrons. The editors invited the authors to make this a very practical book, with specific numerical examples of how experimental data is worked up to produce a distance estimate, and realistic assessments of uncertainties and of the range of applicability, along with examples of the power of the technique to answer biological problems. The first chapter is an overview, by two of the editors, of EPR methods to determine distances, with a focus on the range of applicability. The next chapter, also by the Batons, reviews what is known about electron spin relaxation times that are needed in estimating distances between spins or in selecting appropriate temperatures for particular experiments. Albert Beth and Eric Hustedt describe the information about spin-spin interaction that one can obtain by simulating CW EPR line shapes of nitroxyl radicals. The information in fluid solution CW EPR spectra of dual-spin labeled proteins is illustrated by Hassane Mchaourab and Eduardo Perozo.

Specialist Periodical Reports provide systematic and critical review coverage in major areas of chemical research. Compiled by teams of leading authorities in the relevant subject, the series creates a unique service for the active research chemist with regular critical in-depth accounts of progress in particular areas of chemistry. Subject coverage of all volumes is very similar and publication is on an annual or biennial basis. As EPR continues to find new applications in virtually all areas of modern science, including physics, chemistry, biology and materials science, this series caters not only for experts in the field, but also those wishing to gain a general overview of EPR applications in a given area.

Electron Paramagnetic Resonance (EPR) Volume 19 highlights major developments in this area reported up to the end of 2002, with results being set into the context of earlier work and presented as a set of critical yet coherent overviews. The topics covered describe contrasting types of application, ranging from biological areas such as EPR studies of free-radical reactions in biology and medically-related systems, to experimental developments and applications involving EPR imaging, the use of very high fields, and time-resolved methods. Critical and up-to-the-minute reviews of advances involving the design of spin-traps, advances in spin-labelling, paramagnetic centres on solid surfaces, exchange-coupled oligomers, metalloproteins and radicals in flavoenzymes are also included. As EPR continues to find new applications in virtually all areas of modern science, including physics, chemistry, biology and materials science, this series caters not only for experts in the field, but also those wishing to gain a general overview of EPR applications in a given area. Specialist Periodical Reports provide systematic and detailed review coverage in major areas of chemical research. Compiled by teams of leading authorities in the relevant subject areas, the series creates a unique service for the active research chemist, with regular, in-depth accounts of progress in particular fields of chemistry. Subject coverage within different volumes of a given title is similar and publication is on an annual or biennial basis.

Electron Paramagnetic Resonance (EPR) Volume 18 highlights major developments in this area reported in 2001 and 2002, with results being set into the context of earlier work and presented as a set of critical yet coherent overviews. The topics covered describe contrasting types of application ranging from biological areas such as EPR studies of free-radical reactions in biology and medically-related systems to experimental developments and applications involving EPR imaging the use of very high fields and time-resolved methods. Critical and up-to-the-minute reviews of advances involving the design of spin-traps advances in spin-labelling paramagnetic centres on solid surfaces exchange-coupled oligomers metalloproteins and radicals in flavoenzymes are also included. As EPR continues to find new applications in virtually all areas of modern science including physics chemistry biology and materials science this series caters not only for experts in the field but also those wishing to gain a general overview of EPR applications in a given area.

Electron Paramagnetic Resonance (EPR) Volume 17 highlights major developments in this area reported up to the end of 1999, with results being set into the context of earlier work and presented as a set of critical yet coherent overviews. The topics covered describe contrasting types of application, ranging from biological areas such as EPR and ENDOR studies of metalloproteins and evidence of free-radical reactions in biology and medically-related systems, to experimental developments and applications involving EPR imaging, the use of very high fields, and time-resolved methods. Critical reviews of applications involving bacterial photosynthesis, spin-labelling and spin-probes studies of self-assembled systems, and organometallic chemistry are also included. As EPR continues to find new applications in virtually all areas of modern science, including physics, chemistry, biology and materials science, this series caters not only for experts in the field, but also those wishing to gain a general overview of EPR applications in a given area. Specialist Periodical Reports provide systematic and detailed review coverage in major areas of chemical research. Compiled by teams of leading authorities in the relevant subject areas, the series creates a unique service for the active research chemist, with regular, in-depth accounts of progress in particular fields of chemistry. Subject coverage within different volumes of a given title is similar and publication is on an annual or biennial basis.

Specialist Periodical Reports provide systematic and detailed review coverage of progress in the major areas of chemical research. Written by experts in their specialist fields the series creates a unique service for the active research chemist, supplying regular critical in-depth accounts of progress in particular areas of chemistry. For over 80 years the Royal Society of Chemistry and its predecessor, the Chemical Society, have been publishing reports charting developments in chemistry, which originally took the form of Annual Reports. However, by 1967 the whole spectrum of chemistry could no longer be contained within one volume and the series Specialist Periodical Reports was born. The Annual Reports themselves still existed but were divided into two, and subsequently three, volumes covering Inorganic, Organic and Physical Chemistry. For more general coverage of the highlights in chemistry they remain a 'must'. Since that time the SPR series has altered according to the fluctuating degree of activity in various fields of chemistry. Some titles have remained unchanged, while others have altered their emphasis along with their titles; some have been combined under a new name whereas others have had to be discontinued. The current list of Specialist Periodical Reports can be seen on the inside flap of this volume.