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Distance Measurements in Biological Systems by EPR (Paperback, Softcover reprint of the original 1st ed. 2000)
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Distance Measurements in Biological Systems by EPR (Paperback, Softcover reprint of the original 1st ed. 2000)
Series: Biological Magnetic Resonance, 19
Expected to ship within 10 - 15 working days
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Distance measurements in biological systems by EPR The foundation
for understanding function and dynamics of biological systems is
knowledge of their structure. Many experimental methodologies are
used for determination of structure, each with special utility.
Volumes in this series on Biological Magnetic Resonance emphasize
the methods that involve magnetic resonance. This volume seeks to
provide a critical evaluation of EPR methods for determining the
distances between two unpaired electrons. The editors invited the
authors to make this a very practical book, with specific numerical
examples of how experimental data is worked up to produce a
distance estimate, and realistic assessments of uncertainties and
of the range of applicability, along with examples of the power of
the technique to answer biological problems. The first chapter is
an overview, by two of the editors, of EPR methods to determine
distances, with a focus on the range of applicability. The next
chapter, also by the Batons, reviews what is known about electron
spin relaxation times that are needed in estimating distances
between spins or in selecting appropriate temperatures for
particular experiments. Albert Beth and Eric Hustedt describe the
information about spin-spin interaction that one can obtain by
simulating CW EPR line shapes of nitroxyl radicals. The information
in fluid solution CW EPR spectra of dual-spin labeled proteins is
illustrated by Hassane Mchaourab and Eduardo Perozo.
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