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Aldose 1-epimerase or mutarotase is the enzyme that responsible for
carbohydrate metabolism and converts the alpha anomer into beta
anomer of glucose. This enzyme was extracted from bovine kidney
cortex. Crude enzyme exhibited the activity of 14.92 U mL-1 with
specific activity of 0.153 U mg-1 proteins. The enzyme activity and
specific activity was increased to 53.75 UmL-1 4.981 Umg-1
respectively after 38-60% ammonium sulfate precipitation and it was
further increased to 73.27 UmL-1 and 11.67 Umg-1 when subjected to
diethylaminoethyl (DEAE) cellulose chromatography. Further
purification was carried out by passing it through Sephadex G-150
column and observed increase in activity 79.26 UmL-1 with 19.55
Umg-1 specific activity. The optimum pH and temperature were
recorded as 8.5 and 37 oC respectively. Different stabilizers
(glycerol, sodium benzoate, sodium citrate) were used to study
their effect on stability of enzyme.
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Pregnancy (Paperback)
Shazia Anwer Bukhari, Zia-ur Rahman, Saeed Ahmad
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R1,296
Discovery Miles 12 960
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Ships in 10 - 15 working days
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Serum globulin, total antioxidant status and selenium concentration
decreased significantly while AST, T3, T4, homocysteine, total
oxidant status, MDA, K, Ca, Zn did increase during 1st trimester of
pregnancy. Serum cholesterol, triglyceride, AST, T3, T4,
homocysteine, total oxidant status, MDA, Ca, Zn were significantly
higher while serum total proteins, albumin, antioxidant status, Na,
K and Se did decrease significantly during 2nd trimester. A
significant high level of body temperature, triglyceride,
LDL-cholesterol, AST, T3, homocysteine, total oxidant status, MDA,
Ca and zinc concentration while lower concentration of serum
proteins, albumin, antioxidant status, Na, Fe and Se concentration
was observed in women at the end of the pregnancy. Pearson
correlation was determined to observe the relationship of
homocysteine and DNA damage with the clinical biochemical profiles
of pregnant women.
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Obesity (Paperback)
Shazia Anwer Bukhari, Zia-ur Rahman, Saeed Ahmad
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R1,551
Discovery Miles 15 510
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Ships in 10 - 15 working days
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Forty (40) age matched individuals of male and female, young and
old were selected as normal and obese on the basis of their body
mass index (BMI). A significant increase in concentration of serum
glucose, cholesterol, triglyceride, LDL, albumin, ALT, AST, total
oxidant status, MDA and Hcy and a significant decrease in
concentration of HDL, total proteins, globulins and antioxidant
status (AOS) were observed in obese as compared to normal
individuals. Sodium, potassium, magnesium and manganese
concentration were significantly high and calcium, CU, Zn and Fe
concentration did decrease significantly in obese individuals.
Serum globulin, total antioxidant status and iron concentration
were negative while body mass index, systolic blood pressure,
glucose, cholesterol, triglyceride, LDL, albumin, ALT, total
oxidants status, MDA, sodium and manganese did show a positive
correlation with Hcy in obese individuals. DNA damage was
positively correlated with BMI, systolic blood pressure,
cholesterol, triglycerides, LDL, albumin, ALT, total oxidant
status, MDA, sodium, potassium and magnesium while negative
correlation was observed with globulin, AOS and iron contents
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Diarrhea (Paperback)
Shazia Anwer Bukhari, Zia-ur Rahman, Saeed Ahmad
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R1,292
Discovery Miles 12 920
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Ships in 10 - 15 working days
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Diarrhea a leading cause of illness and death in children in
developing countries may lead to the cause of dehydration resulting
from loss of water and electrolytes and lack of absorption of
nutrients due to inflammatory bowel. A total of forty (40) blood
samples were collected from normal and diarrheal children from
Allied and Civil Hospital, Faisalabad belong to middle
socio-economic class. Blood samples were collected with and without
anticoagulant from each individual for separation of serum which
was presented at -2 C. Serum biochemicals, hormones and health
markers were estimated in triplicate while using standard
procedures of analysis. A significant high concentration of LDL,
ALT, homocysteine, total oxidant status, K and Mg while serum
triglyceride, total proteins, albumin, globulin, T3, T4, total
antioxidant status, Na, Ca, Zn and Cu were significantly low in
patients with diarrhea. Diarrhea patients have shown a significant
increase in DNA damage. In addition, DNA damage has shown a
positive correlation with glucose, LDL and with potassium while
negative correlation was observed with total proteins, thyroxine,
total antioxidant status, sodium and calcium
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