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Rapid Glucose Determination by Mutarotase (Paperback)
Loot Price: R1,064
Discovery Miles 10 640
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Rapid Glucose Determination by Mutarotase (Paperback)
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Aldose 1-epimerase or mutarotase is the enzyme that responsible for
carbohydrate metabolism and converts the alpha anomer into beta
anomer of glucose. This enzyme was extracted from bovine kidney
cortex. Crude enzyme exhibited the activity of 14.92 U mL-1 with
specific activity of 0.153 U mg-1 proteins. The enzyme activity and
specific activity was increased to 53.75 UmL-1 4.981 Umg-1
respectively after 38-60% ammonium sulfate precipitation and it was
further increased to 73.27 UmL-1 and 11.67 Umg-1 when subjected to
diethylaminoethyl (DEAE) cellulose chromatography. Further
purification was carried out by passing it through Sephadex G-150
column and observed increase in activity 79.26 UmL-1 with 19.55
Umg-1 specific activity. The optimum pH and temperature were
recorded as 8.5 and 37 oC respectively. Different stabilizers
(glycerol, sodium benzoate, sodium citrate) were used to study
their effect on stability of enzyme.
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