Die Entwicklung und Einf hrung von Next-Generation Sequencing-Technologien ver ndern die M glichkeiten f r Transkriptomstudien immens. Mit der Leistungsf higkeit der momentanen Sequenziertechnologien k nnen Millionen von Sequenzen in k rzester Zeit sequenziert werden. Mit PASSAGE wird in diesem Buch ein Verfahren zur genomweiten Genexpressionsanalyse ohne ein Referenzgenom vorgestellt. Neben der Analyse von Einzelzellen wird aufgezeigt wie mit PASSAGE auch die Analyse von Metatranskriptomen m glich ist. Ist ein Referenzgenom vorhanden so kann mit PASSAGE der Aufwand der Betrachtung der einzelnen Sequenzen mit eben diesem Verfahren deutlich reduziert werden. Neben der reinen Analyse der Daten wird zudem eine grafische Benutzeroberfl che zur Visualisierung der Ergebnisse vorgestellt. Damit wird dem Anwender eine u erst effiziente Analyse der Daten erm glicht. Der Vergleich mit Microarray-Ergebnissen zeigt die Validit t dieses Verfahrens zur genomweiten Genexpressionsanalyse an Einzelzellen oder von Metatranskriptomen.

This book covers a wide spectrum of connected topics in the field of practical and moral thinking. It contains papers and discussions on the logic of practical reasoning and moral obligation; on the decision-theoretical approach to morality; on the relation between practical and theoretical reason; and on the political aspects of morality. The contributors are, for the most part, well-known philosophers who have not been content with merely restating their positions, but have sued this as a forum for proposing new ideas. The value of the collection lies not only in the originality of the contributions on the various topics mentioned but equally in its demonstration of the interrelations among these topics.

This is an ambitious and substantial study of metaphysics: its nature and inescapability. Professor Koerner's method may be described as 'philosophical anthropology', and aims to arrive at a characterisation of the metaphysical beliefs with which we (have to) operate. Professor Koerner begins by describing how the categorical framework of a person's metaphysical beliefs may be embedded in more ordinary beliefs and practical attitudes to the world. He illustrates the variety of such frameworks and describes their role, going on to explain how they may be modified by argument and reflection. This is an independent inquiry, but also the culmination of a series of Professor Koerner's earlier works. The writing is extremely clear and the material sensitively controlled, revealing great learning and many suggestive insights and comparisons. It presents overall a comprehensive and carefully thought-out account of metaphysics.

First published in 1976, this is a comprehensive study of practical thinking. Professor Koerner shows the complex relations which a person's practical attitudes bear to each other, and shows in particular how their moral or prudential character depends not only on their content and form but also on their place in the system constituted by them. There are detailed accounts of the concepts of morality, prudence, justice, welfare and legality, as well as the logical foundations, epistemology and metaphysics of practical thinking. The book is intended for philosophers and for those political theorists and social scientists who are concerned with the philosophical presuppositions and implications of their enquiries. The book is deliberately organized so that those with less interest in the logical issues dealt with in Part I can proceed quickly and easily to the more substantive issues in Parts II and III.