In this exciting update, discover how feeding direct fed microbials (including eubiotics, postbiotics, prebiotics, and synbiotics) is becoming increasingly widespread during food animal production. Beneficial effects particularly in cattle, pigs, aquaculture, and poultry, including improved general health, foodborne pathogen reduction, more efficient food utilization, increased growth rate and increased milk and egg production. Successes from feeding direct fed microbials in many food animal species which ensures their continual integration into part of a holistic food animal production scheme. Unfortunately, several fundamental questions remain about how and why probiotic approaches succeed, and which kind of probiotic-type products are best for specific production scenarios and schemes (e.g., grass-fed, never-ever antibiotics).  Early establishment and retention of a balanced gastrointestinal microbial ecosystem (non-dysbiotic condition) is critical for a non-native biological additive to be effective in young animals. Therefore, the effectiveness of direct fed microbials in some food animal species may be an indirect consequence of speeding up the establishment and succession of the dominant microflora characteristic of the adult gastrointestinal tract, and preventing a dysbiotic ecosystem from establishing in the gut. We must understand the critical phases and processes involved in microbiota/microbiome establishment in the gut, and how they impact the outputs of the microbial fermentation. By linking the activity of the native and non-native microbial populations and how they interact, we can better design more tailored direct-fed microbials for use at the farm level. Several additional areas of future research directions are also suggested for further development and implementation of next generation direct-fed microbials as advances in sequencing technologies and bioinformatic programs become available. Continued research on direct fed microbials and prebiotics in general should markedly expand their commercial applications, and commercial acceptance and penetration in the animal production market. 

Due to their novel concepts and extraordinary high-throughput sequencing capacity, the "next generation sequencing" methods allow scientists to grasp system-wide landscapes of the complex molecular events taking place in various biological systems, including microorganisms and microbial communities. These methods are now being recognized as essential tools for a more comprehensive and deeper understanding of the mechanisms underlying many biological processes. In High-Throughput Next Generation Sequencing: Methods and Applications, experts in the field explore the most recent advances in the applications of next generation sequencing technologies with an emphasis on microorganisms and their communities; however, the methods described in this book will also offer general applications relevant to the study of any living organisms. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, High-Throughput Next Generation Sequencing: Methods and Applications is an excellent collection of chapters to aid all scientists who wish to apply these innovative research tools to enhance their own pursuits in microbiology and also biology in general.

This comprehensive study of poultry meat safety offers readers the most up-to-date information on food safety concerns in poultry meat production. Chapters address recent topics of interest such as organic poultry production, antimicrobial resistant pathogens in poultry, antibiotic usage in poultry production, and pre- and post- harvest approaches to improving poultry meat safety. The last couple of decades have observed a significant increase in poultry meat production in the US. However, poultry meat is a potential source of foodborne pathogens such as Salmonella, Campylobacter spp. and pathogenic Escherichia coli (APEC linked to human infections), leading to economic losses to the poultry industry and impacting public health. Advances in knowledge in microbiology, molecular biology, immunology and "omics" fields have intensified efforts to improve the microbiological safety of poultry by targeting virulence mechanisms of the pathogens, developing vaccines and improving gut health in chickens. Moreover, due to the emergence of multidrug resistance in poultry-borne pathogens, and growth of organic poultry production, there exists significant interest for developing natural strategies for controlling pathogens in chickens. This edited volume provides insight into these strategies and covers other material of interest to food microbiologists, public health personnel, and poultry scientists. Readers of various backgrounds will appreciate its incorporation of recent developments not covered in other publications on the subject.

This collection features three peer-reviewed literature reviews on pre- and probiotics in poultry production. The first chapter summarises the safety and efficacy of individual monocultures for prophylactic and/or therapeutic efficacy against Salmonella infections in poultry under both laboratory and field conditions. The chapter also considers the development of novel, cost-effective, feed-stable, direct-fed microbials with potential for widespread utilisation and improved production, delivery and clinical efficacy for animal use. The second chapter discusses the establishment of prebiotics as a series of feed compounds that serve as specific substrates for gastrointestinal tract (GIT) bacteria. The chapter reviews the impact of prebiotics on bird health, GIT function, and prevention of foodborne pathogen GIT colonization. Particular attention is drawn on the impact of prebiotics on the avian intestinal microbiome, cecal microbiome and the avian upper GIT. The final chapter considers current concerns surrounding the application of probiotics in poultry production, primarily due to mixed experiences of their effectiveness and lack of knowledge regarding the scientific basis for their modes of action. In contrast, prebiotics have been readily accepted by the sector. This chapter summarises recent research on the effects of both types of treatment, their possible modes of action, as well as the strengths and limitations of their use.

This collection features four peer-reviewed literature reviews on antibiotics in poultry production. The first chapter places the use of antibiotics in poultry production in its historical context to understand the benefits that antibiotics have conferred on animal production to date. It considers past, present and future use of antibiotics, focusing on the use of bacteriocins and phytochemicals. The second chapter considers the benefits and repercussions of the use of antibiotics in poultry production. It details the emergence of antibiotic resistance, as well as the potential risks to public health and sustainable farming associated with antibiotic use. The third chapter reviews the identification and use of prebiotics as a control measure against contamination of poultry products. The chapter covers both traditional prebiotic compounds such as fructooligosaccharides, and less conventional sources such as guar gum. The final chapter presents the background and current use of Competitive Exclusion (CE) as a preventative measure against Salmonella infections in poultry, by administration ofcultures of intestinal origin to day-old chickens.

Due to their novel concepts and extraordinary high-throughput sequencing capacity, the "next generation sequencing" methods allow scientists to grasp system-wide landscapes of the complex molecular events taking place in various biological systems, including microorganisms and microbial communities. These methods are now being recognized as essential tools for a more comprehensive and deeper understanding of the mechanisms underlying many biological processes. In High-Throughput Next Generation Sequencing: Methods and Applications, experts in the field explore the most recent advances in the applications of next generation sequencing technologies with an emphasis on microorganisms and their communities; however, the methods described in this book will also offer general applications relevant to the study of any living organisms. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, High-Throughput Next Generation Sequencing: Methods and Applications is an excellent collection of chapters to aid all scientists who wish to apply these innovative research tools to enhance their own pursuits in microbiology and also biology in general.

"Needless to say, such a book is timely as it provides a valuable account of modern food safety management in poultry processing, coupled with the key elements of sustainable production systems." - Poultry Production To meet growing demand, the FAO has estimated that world poultry production needs to grow by 2-3% per year to 2030. Much of the increase in output already achieved has been as a result of improvements in commercial breeds combined with rearing in more intensive production systems. However, more intensive systems and complex supply chains have increased the risk of rapid transmission of animal diseases and zoonoses. Consumer expectations of sensory and nutritional quality have never been higher. At the same time consumers are more concerned about the environmental impact of poultry production as well as animal welfare. Drawing on an international range of expertise, this book reviews research on safety, quality and sustainability issues in poultry production. Part 1 discusses risks from pathogens, detection and safety management on farms and in slaughterhouse operations. Part 2 looks at ways of enhancing the flavour, colour, texture and nutritional quality of poultry meat. Finally, the book reviews the environmental impact of poultry production. Achieving sustainable production of poultry meat Volume 1: Safety, quality and sustainability will be a standard reference for poultry and food scientists in universities, government and other research centres and companies involved in poultry production. It is accompanied by two further volumes which review poultry breeding, nutrition, health and welfare.

"Improving gut health in poultry is as good for the novice as it is for the experienced nutritionist...this book is a must for every poultry nutritionist."Dr Ioannis Mavromichalis, Ariston Nutrition Consulting International This collection summarises current research on the composition and function of the gastrointestinal tract in poultry, the factors that affect its function, and nutritional strategies to optimise poultry nutrition, health and environmental impact. Part 1 begins by summarising advances in sequencing and omics technologies to understand gut function. It then reviews our current understanding of the gut microbiota, the development of the gut microbiome over the life of the bird, and gut function in nutrient processing and immune response. The second part of the book reviews what we know about factors affecting gut function and health. Chapters cover gastrointestinal diseases, the interaction between pathogens and the gut as well the impact of antibiotics. The final group of chapters discuss current research on the effectiveness of feed additives in optimising gut health, including probiotics, prebiotics, synbiotics, antimicrobials, essential oils and other botanicals as well as cereal grains. With its distinguished editor and team of expert chapter authors, this will be a standard reference for poultry scientists, poultry feed manufacturers and the poultry farming community.

The practice of supplementing direct fed microbial and prebiotic additives to domestic animals during growth is becoming more widespread in food animal production. Beneficial effects particularly in cattle, pigs and poultry, including improved general health, foodborne pathogen reduction, more efficient food utilization, faster growth rate and increased milk and egg production are common results. The success associated with direct fed microbial and prebiotic applications in multiple species ensures their continued commercialization and the widespread use of such additives. However, several fundamental questions remain about how and why probiotic products work, and which kind of probiotic products are best for specific production scenarios.

It appears that early establishment and retention of an ecological balance in the gastrointestinal tract is an important first step for an external biological additive to be effective in young animals. Therefore, it is possible that the effectiveness of direct fed microbials and prebiotics in some animal species may only be an indirect consequence of speeding up the establishment and succession of the dominant microflora characteristic of the adult gastrointestinal tract. Consequently, an understanding of the key processes during establishment of microflora in the gastrointestinal system that lead to the subsequent fermentation characteristics and ecological balance exhibited by the highly protective microflora is needed. Several additional areas of future research directions are also suggested for further development and implementation of these biological approaches as new molecular and drug delivery technologies become available. Continued research on direct fed microbials and prebiotics in general should markedly expand their commercial applications.

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