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Internet of Things
Anurag Upadhyay, Suman Kumar Jha
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R1,569
Discovery Miles 15 690
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Ships in 10 - 15 working days
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Present investigation developed three pathways for in vitro
regeneration and mass multiplication of Acacia mangium. The nodal
segments from 8-10 years old phenotypically superior plus tree were
used as explants for axillary bud culture. MS medium supplemented
with BAP and Kinetin in combination was the best for culture
establishment and shoot proliferation from the axillary buds.
Microshoots were rooted in MS medium (half strength) supplemented
with IBA and acclimatization of the rooted shoots was obtained in
plastic pots containing soilrite. Sixty per cent of cotyledon
explants developed direct adventitious buds. Callus was induced
from the cotyledon and hypocotyl explants on MS medium fortified
with NAA and BAP. Genetic fidelity of micropropagated plants was
examined by RAPD analysis, at every subculture. No genetic
variation was detected among plantlets, hence proving genetically
stable regeneration method. This protocol can be very useful for
for genetic engineering and mass production of quality planting
material of Mangium.
Present investigation was carried out as an effort for genetic
improvement of Dalbergia sissoo. Analysis of variance revealed
significant variation for all the characters under study viz.,
seedling height, collar diameter, dry weight of leaves, leaf area,
nutritional attributes and biochemical attributes.Family no. 25
(Jassur) was found best for nitrogen content, family no. 34
(Khurian) for phosphorus content and family no, 32 (Dhuk) for
potassium content; family no. 28 and 25 were superior for total
sugars and total phenols content. Seedling height showed higher
heritability coupled with high genetic gain. The highly significant
correlation between seedling height and collar diameter, dry weight
of leaves and nitrogen content suggests that these characters can
also be utilized for indirect selection. Cluster analysis showed
distinct clustering pattern.
An efficient in vitro plant regeneration and Agrobacterium
tumefaciens mediated genetic transformation protocol was described
for castor (Ricinus communis L.) using meristem as explants. Shoot
apex containing apical meristems were excised from 5-7 days old in
vitro grown seedlings and cultured on Murashige and Skoog (MS)
medium supplemented with different concentrations of cytokinins
singly or in combination. Kinetin (0.50 mg/l) in combination with
BAP (0.25 mg/l) produced maximum number of shoot (10.33) and shoot
length (5.20 cm). For root induction, in vitro produced shoots were
transferred to rooting media containing 1/2 MS basal media with
NAA. In Agrobacterium tumefaciens mediated genetic transformation,
Agrobacterium tumefaciens strain containing construct pBIN1F
harboring nptII and cry1F gene was used. The integration of the
gene was confirmed by using PCR. The transformation experiment was
performed by optimizing age of the seedlings and co-cultivation
duration with Agrobacterium. When the effect of the age of the
seedlings and co-cultivation duration evaluated fifteen days old
seedlings and co-cultivated for three days yielded the frequency of
transformation 2
Genetic diversity of Karanja accessions were evaluated with Random
Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats
(ISSR) markers. In present study low level of genetic diversity was
observed among the karanj genotypes. Average RPI, PIC and MI value
for RAPD primers were 2.09, 0.25 and 18.06 respectively; while for
ISSR marker it was 0.66, 0.08 and 4.48 respectively. Genetic
diversity analysis by Isozyme marker viz., POX (peroxidase), PPO
(polyphenol oxidase ), EST (esterase) and SOD(superoxide dismutase)
scores total 19 isozyme loci in 30 accessions of Karanja. Highest
loci was scored by PPO (6 loci) followed by POX (5 loci) while 4
loci scored by SOD and EST. Highest polymorphism and highest number
of polymorphic loci was shown by PPO isozyme (83.33%) while lowest
polymorphism and lowest number of polymorphic loci was shown by SOD
isozyme (50 %). In morphological characterization, in terms of
height, tallest tree and average longest branch was recorded by
NAUK-15 and NAUK-16 respectively. Average highest number of
branches and largest leaflet width was recorded by NAUK-9 while
longest leaflet length recorded by NAUK-1.
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